Cytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorDa Silva, Reinaldo J.
dc.creatorSilva, Marcia Guimarães da
dc.creatorVilela, Lízia C.
dc.creatorFecchio, Denise
dc.date2014-05-27T11:20:29Z
dc.date2016-10-25T18:17:54Z
dc.date2014-05-27T11:20:29Z
dc.date2016-10-25T18:17:54Z
dc.date2002-08-01
dc.date.accessioned2017-04-06T01:02:58Z
dc.date.available2017-04-06T01:02:58Z
dc.identifierMediators of Inflammation, v. 11, n. 4, p. 197-201, 2002.
dc.identifier0962-9351
dc.identifierhttp://hdl.handle.net/11449/66946
dc.identifierhttp://acervodigital.unesp.br/handle/11449/66946
dc.identifier10.1080/0962935029000041
dc.identifierWOS:000177984800001
dc.identifier2-s2.0-0036698939.pdf
dc.identifier2-s2.0-0036698939
dc.identifierhttp://dx.doi.org/10.1080/0962935029000041
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/888449
dc.descriptionWE previously demonstrated that Bothrops jararaca venom (BjV) has an antitumor effect on Ehrlich ascites tumor (EAT) cells and induces an increase of polymorphonuclear leukocytes in early stages of tumor growth. It has been reported that this venom presents an important inflammatory effect when inoculated in animal models and in human snake-bites, and that cytokine levels have been detected in these cases. To evaluate whether the cytokines can be involved with the suppression of the tumoral growth, we evaluate the cytokine profile in the peritoneal cavity of mice inoculated with EAT cells and treated with BjV. Swiss mice were inoculated with EAT cells by the intraperitoneal route and treated with BjV venom (0.4 mg/kg, intraperitoneally), on the 1st, 4th, 7th, 10th, and 13th day. Mice were evaluated for cytokine levels on the 2nd, 5th, 8th, 11th and 14th day. Analysis was performed using an enzyme-linked immunosorbent assay for interleukin (IL)-1α, IL-2, IL-4, IL-6, IL-10, IL-13, and tumor necrosis factor-α (TNF-α) levels in the peritoneal washing supernatant. Results were analyzed statistically by the Kruskal-Wallis and Dunn's tests at the 5% level of significance. We observed that EAT implantation induces IL-6 production on the 11th and 14th days of tumor growth, IL-10 on the 11th day and TNF-α on the 14th day. The treatment with BjV suppresses production of these cytokines. In addition, IL-13 was produced by animals that were inoculated only with venom on the 11th and 14th days, and by the group inoculated with EAT cells and treated with venom on the 2nd and 14th days. Furthermore, we suggest that the IL-6 detected in the present study is produced by the EAT cells and the suppression of its production could be associated with the antitumor effect of BjV.
dc.languageeng
dc.relationMediators of Inflammation
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBothrops jararaca
dc.subjectCytokines
dc.subjectEhrlich ascites tumor
dc.subjectSnake venom
dc.subjectbatroxobin
dc.subjectcytokine
dc.subjectinterleukin 10
dc.subjectinterleukin 13
dc.subjectinterleukin 1alpha
dc.subjectinterleukin 2
dc.subjectinterleukin 4
dc.subjectinterleukin 6
dc.subjecttumor necrosis factor alpha
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectantineoplastic activity
dc.subjectcancer inhibition
dc.subjectcontrolled study
dc.subjectenzyme linked immunosorbent assay
dc.subjectmale
dc.subjectmouse
dc.subjectneutrophil
dc.subjectnonhuman
dc.subjectperitoneal cavity
dc.subjectpriority journal
dc.subjecttumor growth
dc.subjectAnimals
dc.subjectCarcinoma, Ehrlich Tumor
dc.subjectCrotalid Venoms
dc.subjectInterleukin-10
dc.subjectInterleukin-13
dc.subjectInterleukin-6
dc.subjectMale
dc.subjectMice
dc.subjectTumor Necrosis Factor-alpha
dc.subjectAnimalia
dc.subjectBothrops
dc.subjectSerpentes
dc.titleCytokine profile of Ehrlich ascites tumor treated with Bothrops jararaca venom
dc.typeOtro


Este ítem pertenece a la siguiente institución