Evaluation of HBsAg and anti-HBc assays in saliva and dried blood spot samples according HIV status

dc.creatorFlores, Geane Lopes
dc.creatorCruz, Helena Medina
dc.creatorPotsch, Denise Vigo
dc.creatorMay, Silvia Beatriz
dc.creatorMello, Carlos Eduardo Brandão
dc.creatorPires, Marcia Maria Amendola
dc.creatorPilotto, José Henrique
dc.creatorLewis-Ximenez, Lia Laura
dc.creatorLampe, Elisabeth
dc.creatorVillar, Livia Melo
dc.date2017-11-09T15:33:45Z
dc.date2017-11-09T15:33:45Z
dc.date2017
dc.date.accessioned2023-09-26T22:53:05Z
dc.date.available2023-09-26T22:53:05Z
dc.identifierFLORES,, Geane Lopes; et al. Evaluation of HBsAg and anti-HBc assays in saliva and dried blood spot samples according HIV status. Journal of Virological Methods, v.247, p.32–37, Sept. 2017.
dc.identifier0166-0934
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23106
dc.identifier10.1016/j.jviromet.2017.05.004
dc.identifier1879-0984
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8884350
dc.descriptionInfluence of HIV status in HBV markers detection in saliva and dried blood spots (DBS) was not well established. This study aims to evaluate the performance of optimized commercial immunoassay for identifying HBsAg and anti-HBc in saliva and DBS according HIV status. A sum of 535 individuals grouped as HIV(+), HBV(+), HIV/HBV(+) and HIV/HBV- were recruited where 347 and 188 were included for HBsAg and anti-HBc evaluation, respectively. Serum, DBS collected in Whatman 903 paper and saliva obtained using salivette device were analyzed using EIA. Increased sample volume and ROC curve analysis for cut off determination were used for DBS and saliva testing. HBsAg detection in saliva and DBS exhibited sensitivities of 80.9% and 85.6% and specificities of 86.8% and 96.3%. Sensitivity of anti-HBc in saliva and DBS were 82.4% and 76.9% and specificities in saliva and DBS were 96.9% and 91.7%. Low sensitivities were observed for HBsAg (62%) and anti-HBc (47%) detection in saliva of HIV/HBV+ individuals. OD values were also lower for HBsAg detection in DBS and saliva of HIV/HBV+ individuals compared to their serum samples. Statistical significance was found for sensitivities in HBsAg detection between saliva and DBS demonstrating high sensitivity for DBS specimens. In conclusion, HIV status or antiretroviral treatment appears to interfere in the performance of HBsAg and anti-HBc detection in DBS and saliva samples using the adapted commercial EIA.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectHIV
dc.subjectHBV
dc.subjectSaliva
dc.subjectPonto de sangue seco
dc.subjectImunoensaio enzimático
dc.subjectHIV
dc.subjectHBV
dc.subjectDried blood spot
dc.subjectSaliva
dc.subjectEnzyme immunoassay
dc.titleEvaluation of HBsAg and anti-HBc assays in saliva and dried blood spot samples according HIV status
dc.typeArticle


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