Brasil | Article

Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation

dc.creatorAbrantes, Tuanne Rotti
dc.creatorMadeira, Maria de Fátima
dc.creatorSilva, Denise Amaro da
dc.creatorPerié, Carolina dos Santos F. S.
dc.creatorMendes Júnior, Artur Augusto V.
dc.creatorMenezes, Rodrigo Caldas
dc.creatorSilva, Valmir Laurentino
dc.creatorFigueiredo, Fabiano Borges
dc.date2019-02-05T11:31:40Z
dc.date2019-02-05T11:31:40Z
dc.date2016
dc.date.accessioned2023-09-26T22:52:36Z
dc.date.available2023-09-26T22:52:36Z
dc.identifierABRANTES, Tuanne Rotti et al. Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, n. 3, p. 1-5, 2016.
dc.identifier0036-4665
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31412
dc.identifier10.1590/S1678-9946201658003
dc.identifier1678-9946
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8884257
dc.descriptionAfter the report of a second case of canine visceral leishmaniasis (CVL) in São Bento da Lagoa, Itaipuaçu, in the municipality of Maricá, Rio de Janeiro State, an epidemiological survey was carried out, through active search, totaling 145 dogs. Indirect immunofluorescence assay (IFA), enzyme-linked immunosorbent assay (ELISA), and rapid chromatographic immunoassay based on dual-path platform (DPP) were used to perform the serological examinations. The parasitological diagnosis of cutaneous fragments was performed by parasitological culture, histopathology, and immunohistochemistry. In the serological assessment, 21 dogs were seropositive by IFA, 17 by ELISA, and 11 by DPP, with sensitivity of 66.7%, 66.7% and 50%, and specificity of 87.2%, 90.2% and 94%, respectively for each technique. The immunohistochemistry of bone marrow using the cell-block technique presented the best results, with six positive dogs found, three of which tested negative by the other parasitological techniques. Leishmania sp. was isolated by parasitological culture in three dogs. The detection of autochthonous Leishmania infantum in Itaipuaçu, and the high prevalence of seropositive dogs confirm the circulation of this parasite in the study area and alert for the risk of expansion in the State of Rio de Janeiro.
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidade de São Paulo
dc.rightsopen access
dc.subjectCanine visceral leishmaniasis
dc.subjectDogs
dc.subjectDiagnosis
dc.subjectCell-block
dc.titleIdentification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation
dc.typeArticle


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