Evidence for involvement of Saccharomyces cerevisiae protein kinase C in glucose induction of HXT genes and derepression of SUC2

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBrandão, R. L.
dc.creatorEtchebehere, L.
dc.creatorQueiroz, C. C.
dc.creatorTrópia, M. J.
dc.creatorErnandes, J. R.
dc.creatorGonçalves, T.
dc.creatorLoureiro-Dias, M. C.
dc.creatorWinderickx, J.
dc.creatorThevelein, J. M.
dc.creatorLeiper, F. C.
dc.creatorCarling, D.
dc.creatorCastro, I. M.
dc.date2014-05-27T11:20:28Z
dc.date2016-10-25T18:17:49Z
dc.date2014-05-27T11:20:28Z
dc.date2016-10-25T18:17:49Z
dc.date2002-06-24
dc.date.accessioned2017-04-06T01:02:41Z
dc.date.available2017-04-06T01:02:41Z
dc.identifierFEMS Yeast Research, v. 2, n. 2, p. 93-102, 2002.
dc.identifier1567-1356
dc.identifierhttp://hdl.handle.net/11449/66910
dc.identifierhttp://acervodigital.unesp.br/handle/11449/66910
dc.identifier10.1016/S1567-1356(02)00077-6
dc.identifierWOS:000178237000003
dc.identifier2-s2.0-0036281358
dc.identifierhttp://dx.doi.org/10.1016/S1567-1356(02)00077-6
dc.identifierhttp://dx.doi.org/10.1111/j.1567-1364.2002.tb00074.x
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/888418
dc.descriptionThe PKC1 gene in the yeast Saccharomyces cerevisiae encodes protein kinase C that is known to control a mitogen-activated protein (MAP) kinase cascade consisting of Bck1, Mkk1 and Mkk2, and Mpk1. This cascade affects the cell wall integrity but the phenotype of Pkc1 mutants suggests additional targets which have not yet been identified. We show that a pkc1Δ mutant, as opposed to mutants in the MAP kinase cascade, displays two major defects in the control of carbon metabolism. It shows a delay in the initiation of fermentation upon addition of glucose and a defect in derepression of SUC2 gene after exhaustion of glucose from the medium. After addition of glucose the production of both ethanol and glycerol started very slowly. The V max of glucose transport dropped considerably and Northern blot analysis showed that induction of the HXT1, HXT2 and HXT4 genes was strongly reduced. Growth of the pkc1Δ mutant was absent on glycerol and poor on galactose and raffinose. Oxygen uptake was barely present. Derepression of invertase activity and SUC2 transcription upon transfer of cells from glucose to raffinose was deficient in the pkc1Δ mutant as opposed to the wild-type. Our results suggest an involvement of Pkc1p in the control of carbon metabolism which is not shared by the downstream MAP kinase cascade. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
dc.languageeng
dc.relationFEMS Yeast Research
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectProtein kinase C
dc.subjectSaccharomyces cerevisiae
dc.subjectSignal transduction
dc.subjectalcohol
dc.subjectfungal enzyme
dc.subjectgalactose
dc.subjectglucose
dc.subjectglycerol
dc.subjectmitogen activated protein kinase
dc.subjectprotein kinase C
dc.subjectraffinose
dc.subjectalcohol production
dc.subjectcarbon metabolism
dc.subjectcell transfer
dc.subjectcontrolled study
dc.subjectdownstream processing
dc.subjectenzyme activity
dc.subjectfermentation
dc.subjectfungal gene
dc.subjectfungus growth
dc.subjectfungus mutant
dc.subjectgene hxt
dc.subjectgene induction
dc.subjectgene repression
dc.subjectgene suc2
dc.subjectgenetic code
dc.subjectgenetic transcription
dc.subjectglucose transport
dc.subjectnonhuman
dc.subjectNorthern blotting
dc.subjectoxygen consumption
dc.subjectwild type
dc.subjectSaccharomyces
dc.titleEvidence for involvement of Saccharomyces cerevisiae protein kinase C in glucose induction of HXT genes and derepression of SUC2
dc.typeOtro


Este ítem pertenece a la siguiente institución