Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates

dc.creatorVilardo, Mônica de Castro Britto
dc.creatorThomé, Jacqueline Darc da Silva
dc.creatorEsteves, Wagner Thadeu Cardoso
dc.creatorFilgueiras, Ana Luzia Lauria
dc.creatorOliveira, Selma Soares de
dc.date2020-02-06T12:18:06Z
dc.date2020-02-06T12:18:06Z
dc.date2006
dc.date.accessioned2023-09-26T22:51:21Z
dc.date.available2023-09-26T22:51:21Z
dc.identifierVILARDO, Mônica de Castro Brito et al. Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 5, p. 499-501, Aug. 2006.
dc.identifier0074-0206
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/39762
dc.identifier10.1590/s0074-02762006000500003
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8884000
dc.descriptionA multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectCampylobacter
dc.subjectIdentificação
dc.subjectPrimatas não-humanos
dc.subjectCampylobacter
dc.subjectIdentification
dc.subjectNon-human primates
dc.titleApplication of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
dc.typeArticle


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