Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud

dc.creatorBraga, Maria Flaviana Bezerra Morais
dc.creatorLeal, Antonio Linkoln Alves Borges
dc.creatorOliveira, Alison Honorio de
dc.creatorCarneiro, Joara N alyda Pereira
dc.creatorSantos, Antonia Thassya Lucas dos
dc.creatorSales, Débora Lima
dc.creatorFonseca, Victor Juno Alencar
dc.creatorRocha, Janaina Esmeraldo
dc.creatorFreitas, Thiago Sampaio de
dc.creatorCampina, Fábia Ferreira
dc.creatorCosta, Maria do Socorro
dc.creatorPereira Junior, Francisco Nascimento
dc.creatorAmaral, Wanderlei do
dc.creatorRebelo, Ricardo Andrade
dc.creatorSilva, Luiz Everson da
dc.creatorRibeiro Filho, Jaime
dc.creatorCoutinho, Henrique Douglas Melo
dc.date2022-04-06T16:55:03Z
dc.date2022-04-06T16:55:03Z
dc.date2022
dc.date.accessioned2023-09-26T22:49:51Z
dc.date.available2023-09-26T22:49:51Z
dc.identifierBRAGA, Maria Flaviana Bezerra Morais et al. Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud. South African Journal of Botany, p. 1-9, 2022.
dc.identifier0254-6299
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/52061
dc.identifier10.1016/j.sajb.2022.03.017
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8883719
dc.descriptionFundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico - FUNCAP
dc.descriptionAntimicrobial resistance has emerged as a health public concern as the combat of resistant microorganisms has become increasingly challenging. Thus, considering the urgent need of developing new therapeutic alternatives, the present study aimed to investigate the chemical composition and evaluate the antimicrobial and drug enhancing potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud (EOLPx) against clinically relevant bacterial and fungal strains. Following collection, the leaves were dried at room temperature in a dark environment and the extraction was performed by hydrodistillation using a modified Clevenger-type apparatus. The oil was dried with anhydrous magnesium sulfate, filtered, concentrated in rotary evaporator, and 5 mL aliquots were stored in a refrigerator. The oil yield was expressed as a percentage of the dry weight of the plant material (w/w). The chemical characterization was performed by gas chromatography coupled to mass spectrometry (GC–MS) and flame ionization detector (FID). The antimicrobial analysis was carried out using the broth microdilution and half-maximal inhibitory concentrations (IC50), minimum inhibitory concentrations (MIC), and minimum fungicidal concentrations (MFC) were deterimined by subculture. To evaluate the drug enhancing potential, subinhibitory concentrations (MFC/16 and MIC/8) of the essential oil were combined with the antifungal drug fluconazole and the antibiotics norfloxacin, gentamicin and erythromycin. The impact of the in vitro treatment on fungal virulence was determined by observing morphological transition in wet chambers. The chemical analysis identified the presence of monoterpenes (58.8%) and sesquiterpenes (35.3%), including the monoterpene hydrocarbon myrcene (27.5%) as the major component. The in vitro treatment with the essential oil caused little impact on C. albicans growth, showing a weak fungicidal effect against the URM 4387 strain (at 8192 µg/mL), while the INCQS 40006 strain was not affected. In addition, the association of the EOLPx with fluconazole did not enhance the antifungal effect of this drug against none of the C. albicans strains. However, both the natural product and fluconazole inhibited fungal pleomorphism by both strains. While the essential oil MIC against all bacterial strains was higher than 813 μg/mL, it was found to enhance the activity of gentamicin against the SA and EC isolates as well as to potentiate the activity of erythromycin against the EC isolate. On the other hand, antagonistic effects were observed from its association with norfloxacin for both strains. In conclusion, the EOLPx showed antibiotic-enhancing effects in addition to inhibiting C. albicans morphological transition. Nevertheless, further research is required to better characterize the antimicrobial effects and mechanisms of action by the essential oil components.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectPiperaceae
dc.subjectÓleos voláteis
dc.subjectFolhas de planta
dc.subjectAnti-Infecciosos
dc.subjectPiperaceae
dc.subjectOils, volatile
dc.subjectPlant leaves
dc.subjectAnti-Infective Agents
dc.subjectPiperaceae
dc.subjectAceites volátiles
dc.subjectHojas de la planta
dc.subjectAntiinfecciosos
dc.subjectPiperaceae
dc.subjectHuile essentielle
dc.subjectFeuilles de plante
dc.subjectAnti-infectieux
dc.titleChemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud
dc.typeArticle


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