Leishmania mexicana amazonensis: heterogeneity in 5'-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection

dc.creatorCôrte-Real, Suzana
dc.creatorGrimaldi Juńior, Gabriel
dc.creatorMeirelles, Maria de Nazareth Leal de
dc.date2019-11-12T12:16:13Z
dc.date2019-11-12T12:16:13Z
dc.date1988
dc.date.accessioned2023-09-26T22:49:28Z
dc.date.available2023-09-26T22:49:28Z
dc.identifierCÔRTE-REAL, Suzana et al. Leishmania mexicana amazonensis: heterogeneity in 5-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 83, n. 1, p. 113-121, Jan./Mar. 1988.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36992
dc.identifier10.1590/s0074-02761988000100015
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8883640
dc.descriptionUm estudo sobre o grau de maturação das células do Sistema Fagocítico Mononuclear foi realizado durante a infecção in vivo e in vitro com a Leishmania mexicana amazonensis. A caracterização da diferenciação das células fagocíticas foi obtida com a localização ultraestrutural de dois marcadores enzimáticos bam conhecidos: a enzima 5'-Nucleotidase marcadora de membrana plasmática de células maduras e a enzima peroxidase, presente em grânulos, marcadora de células imaturas. A atividade da enzima 5'-Nucleotidase foi encontrada apenas em alguns macrófagos, presentes no foco inflamatório, em projeções da membrana plasmática e em algumas vesículas citoplasmáticas. Macrófagos peritoneais de camundongo apresentaram a mesma reatividade para este marcador. Contudo a análise da atividade peroxidásica demonstrou a predominância da presença de fagócitos mononucleares imaturos nas lesões crônicas induzidas neste sistema por Leishmania mexicana amazonensis.
dc.descriptionThe degree of maturation of cells of the Mononuclear Phagocyte System (MPS), during in vivo and in vitro infection by Leishmania mexicana amazonensis, was evaluated in this study. The macrophages' differentiation was assayed by cytochemical characterization at the ultrastructural level, using two well-established markers: 5'-nucleotidase enzyme activity, for revealing the mature cells; and the peroxidase activity present in the cell granules to demonstrate immature mononuclear phagocytes. Only a few macrophages, demonstrating 5'-nucleotidase positive reaction in both the plasma membrane and within their cytoplasmic vesicles, were found scattered in the chronic inflammation at the L. m. amazonensis lesions in albino mice. However, by the peroxidase activity analysis, we were also able to demonstrate the presence of immature MPS cells, which predominate, together with parasitized vacuolated macrophages, in chronic lesions induced in this system by L. m. amazonensis. The implications of these results on the pathogenesis of murine cutaneous leishmaniasis are discussed.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectLeishmania mexicana amazonensis (L. m. amazonensis)
dc.subjectFagócito mononuclear imaturo
dc.subjectMacrófago maturo
dc.subjectMacrófago peritoneal
dc.subjectLesões cutâneas
dc.subject5'Nucleotidase (E. C. 3. 1. 3. 5)
dc.subjectPeroxidase (E. C. 1. 11. 1. 7)
dc.subjectLeishmania mexicana amazonensis (L. m. amazonensis)
dc.subjectPeroxidase (E. C. 1. 11. 1. 7)
dc.subject5'Nucleotidase (E. C. 3. 1. 3. 5)
dc.subjectImmature mononuclear phagocyte
dc.subjectMature macrophages
dc.subjectPeriotoneal macrophages
dc.subjectCutaneous lesions
dc.titleLeishmania mexicana amazonensis: heterogeneity in 5'-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection
dc.typeArticle


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