B-Cell Epitope Mapping of the Plasmodium falciparum Malaria Vaccine Candidate GMZ2.6c in a Naturally Exposed Population of the Brazilian Amazon

dc.creatorBaptista, Barbara de Oliveira
dc.creatorSouza, Ana Beatriz Lopes de
dc.creatorOliveira, Luana Santos de
dc.creatorSouza, Hugo Amorim dos Santos de
dc.creatorBarros, Jenifer Peixoto de
dc.creatorQueiroz, Lucas Tavares de
dc.creatorSouza, Rodrigo Medeiros de
dc.creatorAmoah, Linda Eva
dc.creatorSingh, Susheel Kumar
dc.creatorTheisen, Michael
dc.creatorSilva, Rodrigo Nunes Rodrigues da
dc.creatorRiccio, Evelyn Kety Pratt
dc.creatorTotino, Paulo Renato Rivas
dc.creatorLIma Junior, Josué da Costa
dc.creatorRibeiro, Cláudio Tadeu Daniel
dc.creatorPratt-Riccio, Lilian Rose
dc.date2023-03-23T16:25:20Z
dc.date2023-03-23T16:25:20Z
dc.date2023
dc.date.accessioned2023-09-26T22:49:26Z
dc.date.available2023-09-26T22:49:26Z
dc.identifierBAPTISTA, Barbara de Oliveira et al. B-Cell Epitope Mapping of the Plasmodium falciparum Malaria Vaccine Candidate GMZ2.6c in a Naturally Exposed Population of the Brazilian Amazon. Vaccines, v.11, 446, p. 1 - 17, Feb. 2023.
dc.identifier2076-393X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/57536
dc.identifier10.3390/vaccines11020446
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8883633
dc.descriptionThe GMZ2.6c malaria vaccine candidate is a multi-stage P. falciparum chimeric protein that contains a fragment of the sexual-stage Pf s48/45-6C protein genetically fused to GMZ2, an asexualstage vaccine construction consisting of the N-terminal region of the glutamate-rich protein (GLURP) and the C-terminal region of the merozoite surface protein-3 (MSP-3). Previous studies showed that GMZ2.6c is widely recognized by antibodies from Brazilian exposed individuals and that its components are immunogenic in natural infection by P. falciparum. In addition, anti-GMZ2.6c antibodies increase with exposure to infection and may contribute to parasite immunity. Therefore, identifying epitopes of proteins recognized by antibodies may be an important tool for understanding protective immunity. Herein, we identify and validate the B-cell epitopes of GMZ2.6c as immunogenic and immunodominant in individuals exposed to malaria living in endemic areas of the Brazilian Amazon. Specific IgG antibodies and subclasses against MSP-3, GLURP, and Pfs48/45 epitopes were detected by ELISA using synthetic peptides corresponding to B-cell epitopes previously described for MSP-3 and GLURP or identified by BepiPred for Pf s48/45. The results showed that the immunodominant epitopes were P11 from GLURP and MSP-3c and DG210 from MSP-3. The IgG1 and IgG3 subclasses were preferentially induced against these epitopes, supporting previous studies that these proteins are targets for cytophilic antibodies, important for the acquisition of protective immunity. Most individuals presented detectable IgG antibodies against Pf s48/45a and/or Pf s48/45b, validating the prediction of linear B-cell epitopes. The higher frequency and antibody levels against different epitopes from GLURP, MSP-3, and Pf s48/45 provide additional information that may suggest the relevance of GMZ2.6c as a multi-stage malaria vaccine candidate.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectGMZ2.6c
dc.subjectMapeamento de epítopos
dc.subjectAnticorpos
dc.subjectMalária
dc.subjectPlasmodium falciparum
dc.subjectVacina
dc.subjectGMZ2.6c
dc.subjectEpitope mapping
dc.subjectAntibodies
dc.subjectMalaria
dc.subjectPlasmodium falciparum
dc.subjectVaccine
dc.titleB-Cell Epitope Mapping of the Plasmodium falciparum Malaria Vaccine Candidate GMZ2.6c in a Naturally Exposed Population of the Brazilian Amazon
dc.typeArticle


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