An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)

dc.creatorFernandes, Octávio
dc.creatorBozza, Marcelo
dc.creatorPascale, Juan M.
dc.creatorMiranda, Antonio B. de
dc.creatorLopes, Ulisses G.
dc.creatorDegrave, Wim M.
dc.date2019-11-02T19:31:21Z
dc.date2019-11-02T19:31:21Z
dc.date1996
dc.date.accessioned2023-09-26T22:49:03Z
dc.date.available2023-09-26T22:49:03Z
dc.identifierFERNANDES, Octavio et al. An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 91, n. 3, p. 279-284, May/June 1996.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36812
dc.identifier10.1590/S0074-02761996000300005
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8883553
dc.descriptionSequence analysis of Leishmania (Viannia) kDNA minicircles and analysis of multiple sequence alignments of the conserved region (minirepeats) of five distinct minicircles from L. (V.) braziliensis species with corresponding sequences derived from other dermotropic leishmanias indicated the presence of a sub-genus specific sequence. An oligonucleotide bearing this sequence was designed and used as a molecular probe, being able to recognize solely the sub-genus Viannia species in hybridization experiments. A dendrogram reflecting the homologies among the minirepeat sequences was constructed. Sequence clustering was obtained corresponding to the traditional classification based on similarity of biochemical, biological and parasitological characteristics of these Leishmania species, distinguishing the Old World dermotropic leishmanias, the New World dermotropic leishmanias of the sub-genus Leishmania and of the sub-genus Viannia.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectLeishmania braziliensis
dc.subjectLeishmania panamensis
dc.subjectLeishmania (Viannia) identification
dc.subjectMinicircle DNA sequences
dc.subjectOligonucleotide probe
dc.subjectLeishmania braziliensis
dc.subjectLeishmania panamensis
dc.titleAn oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
dc.typeArticle


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