Brasil | Article

Genotoxic activity of L-asparaginase produced by Streptomyces ansochromogenes UFPEDA 3420

dc.creatorLacerda, Glêzia Renata da Silva
dc.creatorCantalice, Jeanne Cristina Lapenda Lins
dc.creatorLima, Gláucia Manoella de Souza
dc.creatorAlbuquerque, Luiz Eduardo Félix de
dc.creatorSilva, Isllan D'Erik Gonçalves da
dc.creatorMelo, Maria Eliane Bezerra de
dc.creatorAdam, Mônica Lúcia
dc.creatorNascimento, Silene Carneiro do
dc.date2019-10-03T17:13:40Z
dc.date2019-10-03T17:13:40Z
dc.date2019
dc.date.accessioned2023-09-26T22:48:44Z
dc.date.available2023-09-26T22:48:44Z
dc.identifierLACERDA, Glêzia Renata da Silva et al. Genotoxic activity of L-asparaginase produced by Streptomyces ansochromogenes UFPEDA 3420. World Journal of Microbiology and Biotechnology, v. 35, n. 3, p. 1-9, Feb. 2019.
dc.identifier0959-3993
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36153
dc.identifier10.1007/s11274-019-2612-2
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8883486
dc.descriptionL-asparaginase is an enzyme capable of hydrolyzing the substrate asparagine in aspartic acid and ammonia. Due to this mechanism of action observed, L-asparaginase is widely used in the treatment of Acute Lymphoblastic Leukemia, since these cells use asparagine for their survival. Because it is frequently used as an antineoplastic, it is necessary to evaluate its genotoxic effects. The aim of the present study was to evaluate cellular DNA damage after exposure to L-asparaginase produced by Streptomyces ansochromogenes UFPEDA 3420. NCIH-292, MCF-7 and MOLT-4 neoplastic cell lines and normal PBMC cells were used. L-Asparaginase used in this study was produced by actinobacteria S. ansochromogenes UFPEDA 3420, isolated and purified by chromatographic methods. L-Asparaginase induced micronucleus formation in PBMC cells and tumor lines when compared to the negative control. These data suggest that L-Asp appears to have a genotoxic effect very close to the positive control in normal cells (p < 0.05). The level of genomic damage measured by DNA breaks in alkaline SCGE assay was detected from the lowest concentration (12.5 µg/mL) to the highest concentration (50 µg/mL) for tumor cell lines and PBMC. In view of the above, new genotoxic studies will be carried out to better elucidate L-Asparaginase and its mutagenic potential, still unknown, enough for this drug to be safely used in conventional antineoplastic therapies.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectL-Asparaginase
dc.subjectGenotoxicity
dc.subjectHuman and normal tumor cell lines
dc.subjectAntineoplásicos / farmacologia
dc.subjectAsparaginase / isolamento & purificação
dc.subjectAsparaginase / farmacologia
dc.subjectAsparagina / metabolismo
dc.subjectÁcido Aspártico / metabolismo
dc.subjectLinhagem Celular Tumoral / efeitos dos fármacos
dc.subjectDano ao DNA / efeitos dos fármacos
dc.subjectEnsaios Enzimáticos
dc.subjectHumanos
dc.subjectConcentração Inibidora 50
dc.subjectLeucócitos Mononucleares / efeitos dos fármacos
dc.subjectTestes para Micronúcleos
dc.subjectStreptomyces / enzimologia
dc.subjectStreptomyces / metabolismo
dc.titleGenotoxic activity of L-asparaginase produced by Streptomyces ansochromogenes UFPEDA 3420
dc.typeArticle


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