Brasil | Article

Immunological diagnosis of tuberculosis based on recombinant antigens ESAT-6 and CFP-10 in children from an endemic area in northeast Brazil

dc.creatorVan-Lume, D. S. M.
dc.creatorSouza, J. R. de
dc.creatorCabral, M. M. L.
dc.creatorRego, J. C.
dc.creatorBalbino, V.
dc.creatorSaad, M. H.
dc.creatorSchindler, H. C.
dc.creatorAbath, Frederico Guilherme Coutinho
dc.creatorMontenegro, S. M. L.
dc.date2018-04-25T14:15:25Z
dc.date2018-04-25T14:15:25Z
dc.date2010
dc.date.accessioned2023-09-26T22:48:40Z
dc.date.available2023-09-26T22:48:40Z
dc.identifierVAN-LUME, D. S. M. et al. Immunological diagnosis of tuberculosis based on recombinant antigens ESAT-6 and CFP-10 in children from an endemic area in northeast Brazil. Scandinavian Journal of Immunology, v. 72, n. 5, p. 460–468, nov. 2010.
dc.identifier1365-3083
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26091
dc.identifier10.1111/j.1365-3083.2010.02459.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8883473
dc.descriptionPDTIS (Programa de Desenvolvimento Tecnológico de Saúde) / FIOCRUZ e CAPES.
dc.descriptionDiagnostic tests for tuberculosis (TB) using interferon gamma (IFN-γ) responses produced by T lymphocytes after stimulation by early secretory antigen target 6 (ESAT-6), culture filtrate protein 10 (CFP-10) or purified protein derivate (PPD) were carried out using ELISA (enzyme-linked immunosorbent assay) in whole blood culture supernatants from children with suspected TB disease (n=21), latent TB infection (LTBI; n=17) and negative controls (NC; n=21) from Recife, Pernambuco, Brazil. The results were analysed using the ROC (receiver operating characteristic) curves and the areas under the curve (AUC) generated varied from 0.5 to 1.0 with higher values indicating increased discriminatory ability. Comparisons of AUCs were made using non-parametric assumptions, and the differences were considered significant if P<0.05. The ROC curve showed a statistical difference (P = 0.015) between the LTBI and NC groups with an AUC of 0.731, TB disease and NC (AUC=0.780; P=0.002) and a group with TB (latent infection+disease, n=38) and NC (AUC=0.758; P = 0.001) when the antigen used was ESAT-6. No statistical difference was found between the groups when CFP-10 or PPD was used. In conclusion, the ESAT-6 test may be the most appropriate for diagnosis of childhood TB, both LTBI and TB disease, when associated with epidemiological and clinical data, especially in endemic areas such as Brazil.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectDiagnóstico
dc.subjectTuberculose
dc.subjectDoenças endêmicas
dc.subjectDiagnosis
dc.subjectTuberculosis
dc.subjectEndemic diseases
dc.subjectAdolescente
dc.subjectAntígenos Bacterianos / Genéticos
dc.subjectAntígenos Bacterianos / imunologia
dc.subjectProteínas Bacterianas / genética
dc.subjectProteínas Bacterianas / imunologia
dc.subjectBrasil / epidemiologia
dc.subjectCriança
dc.subjectCriança, pré-escolar
dc.subjectDoenças endêmicas
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectFêmea
dc.subjectHumanos
dc.subjectInterferon-gamma / metabolismo
dc.subjectMasculino
dc.subjectProteínas Recombinantes / imunologia
dc.subjectSensibilidade e Especificidade
dc.subjectLinfócitos T / imunologia
dc.subjectLinfócitos T / metabolismo
dc.subjectTuberculose / diagnóstico
dc.subjectTuberculose / epidemiologia
dc.subjectTuberculose / imunologia
dc.titleImmunological diagnosis of tuberculosis based on recombinant antigens ESAT-6 and CFP-10 in children from an endemic area in northeast Brazil
dc.typeArticle


Este ítem pertenece a la siguiente institución