Characterization of Constitutive and Putative Differentially Expressed mRNAs by Means of Expressed Sequence Tags, Differential Display Reverse Transcriptase-PCR and Randomly Amplified Polymorphic DNA-PCR from the Sand Fly Vector Lutzomyia longipalpis

dc.creatorOrtigão, J. M. Ramalho
dc.creatorTemporal, P.
dc.creatorOliveira, S. M. P.
dc.creatorBarbosa, A. F.
dc.creatorVilela, M. L.
dc.creatorRangel, Elizabeth F.
dc.creatorBrazil, R. P.
dc.creatorTraub-Cseko, Y. M.
dc.date2020-10-21T20:15:04Z
dc.date2020-10-21T20:15:04Z
dc.date2001
dc.date.accessioned2023-09-26T22:48:07Z
dc.date.available2023-09-26T22:48:07Z
dc.identifierORTIGÃO, J. M. Ramalho et al. Characterization of Constitutive and Putative Differentially Expressed mRNAs by Means of Expressed Sequence Tags, Differential Display Reverse Transcriptase-PCR and Randomly Amplified Polymorphic DNA-PCR from the Sand Fly Vector Lutzomyia longipalpis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 1, p. 105-111, Jan. 2001.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44110
dc.identifier10.1590/S0074-02762001000100012
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8883359
dc.descriptionMolecular studies of insect disease vectors are of paramount importance for understanding parasite-vector relationship. Advances in this area have led to important findings regarding changes in vectors’ physiology upon blood feeding and parasite infection. Mechanisms for interfering with the vectorial capacity of insects responsible for the transmission of diseases such as malaria, Chagas disease and dengue fever are being devised with the ultimate goal of developing transgenic insects. A primary necessity for this goal is information on gene expression and control in the target insect. Our group is investigating molecular aspects of the interaction between Leishmania parasites and Lutzomyia sand flies. As an initial step in our studies we have used random sequencing of cDNA clones from two expression libraries made from head/thorax and abdomen of sugar fed L. longipalpis for the identification of expressed sequence tags (EST). We applied differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR to characterize differentially expressed mRNA from sugar and blood fed insects, and, in one case, from a L. (V.) braziliensis-infected L. longipalpis. We identified 37 cDNAs that have shown homology to known sequences from GeneBank. Of these, 32 cDNAs code for constitutive proteins such as zinc finger protein, glutamine synthetase, G binding protein, ubiquitin conjugating enzyme. Three are putative differentially expressed cDNAs from blood fed and Leishmaniainfected midgut, a chitinase, a V-ATPase and a MAP kinase. Finally, two sequences are homologous to Drosophila melanogaster gene products recently discovered through the Drosophila genome initiative.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectLutzomyia longipalpis
dc.subjectLeishmania
dc.subjectPsychodidae
dc.subjectApresentação de Dados
dc.subjectTags de sequência expressa
dc.subjectSand fly
dc.subjectLutzomyia longipalpis
dc.subjectDifferential
dc.subjectLeishmania
dc.subjectDisplay
dc.subjectExpressed sequence tags
dc.subjectApresentação de Dados
dc.titleCharacterization of Constitutive and Putative Differentially Expressed mRNAs by Means of Expressed Sequence Tags, Differential Display Reverse Transcriptase-PCR and Randomly Amplified Polymorphic DNA-PCR from the Sand Fly Vector Lutzomyia longipalpis
dc.typeArticle


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