Lipophosphoglycans from dermotropic Leishmania infantum are more pro-inflammatory than those from viscerotropic strains

dc.creatorCardoso, Camila Almeida
dc.creatorAraujo, Gabriela V
dc.creatorSandoval, Carmen M
dc.creatorNogueira, Paula Monalisa
dc.creatorZúniga, Concepcion
dc.creatorSosa-Ochoa, Wilfredo H
dc.creatorLaurenti, Márcia D
dc.creatorSoares, Rodrigo Pedro Pinto
dc.date2020-10-20T15:51:30Z
dc.date2020-10-20T15:51:30Z
dc.date2020
dc.date.accessioned2023-09-26T22:47:41Z
dc.date.available2023-09-26T22:47:41Z
dc.identifierCARDOSO, Camila Almeida et al. Lipophosphoglycans from dermotropic Leishmania infantum are more pro-inflammatory than those from viscerotropic strains. Mem. Inst. Oswaldo Cruz, v. 115, e200140, 2020
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44080
dc.identifier10.1590/0074-02760200140
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8883269
dc.descriptionAlthough Leishmania infantum is well-known as the aethiological agent of visceral leishmaniasis (VL), in some Central American countries it may cause atypical non-ulcerated cutaneous leishmaniasis (NUCL). However, the mechanisms favoring its establishment in the skin are still unknown. Lipophosphoglycan (LPG) is the major Leishmania multivirulence factor involved in parasite-host interaction. In the case of viscerotropic L. infantum, it causes an immunosuppression during the interaction with macrophages. Here, we investigated the biochemical and functional roles of LPGs from four dermotropic L. infantum strains from Honduras during in vitro interaction with murine macrophages. LPGs were extracted, purified and their repeat units analysed. They did not have side chains consisting of Gal(β1,4)Man(α1)-PO4 common to all LPGs. Peritoneal macrophages from BALB/c and C57BL/6 were exposed to LPG for nitric oxide (NO) and cytokine (TNF-α and, IL-6) production. LPGs from dermotropic strains from Honduras triggered higher NO and cytokine levels compared to those from viscerotropic strains. In conclusion, LPGs from dermotropic strains are devoid of side-chains and exhibit high pro-inflammatory activity.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz
dc.rightsopen access
dc.subjectlipophosphoglycan
dc.subjectLeishmania infantum
dc.subjectinnate immunity
dc.titleLipophosphoglycans from dermotropic Leishmania infantum are more pro-inflammatory than those from viscerotropic strains
dc.typeArticle


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