Transcriptomic profile of two developmental stages of the cestode parasite Mesocestoides corti

dc.creatorCabrera, Tatiana Noel Basika
dc.creatorPaludo, G. P.
dc.creatorAraujo, Flavio Marcos
dc.creatorSalim, Anna Christina de Matos
dc.creatorPais, Fabiano Sviatopolk Mirsky
dc.creatorMaldonado, L.
dc.creatorMacchiaroli, N.
dc.creatorLima, J. Camargo de
dc.creatorRosenzvit, M.
dc.creatorOliveira, Guilherme Corrêa de
dc.creatorKamenetzky, L.
dc.creatorFerreira, Henrique Bunselmeyer
dc.date2019-10-10T17:14:53Z
dc.date2019-10-10T17:14:53Z
dc.date2019
dc.date.accessioned2023-09-26T22:46:50Z
dc.date.available2023-09-26T22:46:50Z
dc.identifierCABRERA, Tatiana Noel Basika et al. Transcriptomic profile of two developmental stages of the cestode parasite Mesocestoides corti. Molecular and Biochemical Parasitology, v. 229, p. 35-46, 2019.
dc.identifier0166-6851
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36354
dc.identifier10.1016/j.molbiopara.2019.02.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8883107
dc.descriptionCestode development involves complex morphological and physiological changes. Here, we performed a differential expression analysis of gene transcripts between two developmental stages of the model cestode Mesocestoides corti. A RNA-seq-based approach was used to compare the transcriptomes of the tetrathyridium (larval, TT) and strobilated worm (ST) stages of the parasite. We found 19,053 transcripts, from which ∼45% were complete matches to genes previously annotated in the available M. corti draft genome sequence, ∼24% were considered novel isoforms, and ∼24% were considered potential novel transcripts. Stage-specific transcripts were found for both TTs (66) and STs (136), along with shared transcripts significantly overrepresented in one stage (342 in TTs, and 559 in STs). Differential expression and Gene Ontology term enrichment analyzes provided evidence of upregulation of different sets of transcripts associated with ‘cytoskeleton’, ‘metabolism’ and ‘oxidation-reduction’ processes in each stage, suggesting functional involvement of the corresponding genes with stage-specific features. Transcripts and processes enriched in the TT reflect typical larval processes that occur with the parasite in the intermediate host, such as asexual reproduction and budding, as well as active migration from the peritoneum to the liver and vice versa. In STs, transcripts associated with ‘development’, ‘cell growth’, and ‘morphogenesis’ were enriched, along with processes related to sexual reproduction, represented by the upregulation of numerous transcription factors, protein kinases, and histones. Overall, our results contributed to significantly increase the knowledge on the M. corti gene repertoire and expression profile in two developmental stages. Functional implications for the biology of larval and adult cestode parasites and for host-parasite interactions are discussed.
dc.description2100-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier/North-Holland Biomedical Press
dc.rightsrestricted access
dc.subjectRNA-seq
dc.subjectDEG
dc.subjectTrascriptome
dc.subjectStrobilation
dc.subjectSegmentation
dc.subjectCestoda
dc.titleTranscriptomic profile of two developmental stages of the cestode parasite Mesocestoides corti
dc.typeArticle


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