Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel

dc.creatorSantos, Luciane Amorim
dc.creatorAdhikarla, Haritha
dc.creatorYan, Xiting
dc.creatorWang, Zheng
dc.creatorFouts, Derrick E
dc.creatorVinetz, Joseph M
dc.creatorAlcantara, Luiz Carlos Júnior
dc.creatorHartskeerl, Rudy A
dc.creatorGoris, Marga G. A
dc.creatorPicardeau, Mathieu
dc.creatorReis, Mitermayer Galvão dos
dc.creatorTownsend, Jeffrey P
dc.creatorZhao, Hongyu
dc.creatorKo, Albert Icksang
dc.creatorWunder Junior, Elsio Augusto
dc.date2018-07-12T16:27:00Z
dc.date2018-07-12T16:27:00Z
dc.date2018
dc.date.accessioned2023-09-26T22:46:42Z
dc.date.available2023-09-26T22:46:42Z
dc.identifierSANTOS, L. A. et al. Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel. Frontiers in Cellular and Infection Microbiology, v. 8, art. 193, 2018.
dc.identifier2235-2988
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27510
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8883079
dc.descriptionPrograma Ciências sem fronteiras Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq) of Brazil and Brasil SemMiséria Program of the Coordenação de Aperfeiçoamento de Pessoal de Nível Superios (Capes) of Brazil.
dc.descriptionLeptospirosis is a worldwide zoonosis, responsible for more than 1 million cases and 60,000 deaths every year. Among the 13 pathogenic species of the genus Leptospira, serovars belonging to L. interrogans serogroup Icterohaemorrhagiae are considered to be the most virulent strains, and responsible for majority of the reported severe cases. Serovars Copenhageni and Icterohaemorrhagiae are major representatives of this serogroup and despite their public health relevance, little is known regarding the genetic differences between these two serovars. In this study, we analyzed the genome sequences of 67 isolates belonging to L. interrogans serovars Copenhageni and Icterohaemorrhagiae to investigate the influence of spatial and temporal variations on DNA sequence diversity. Out of the 1072 SNPs identified, 276 were in non-coding regions and 796 in coding regions. Indel analyses identified 258 indels, out of which 191 were found in coding regions and 67 in non-coding regions. Our phylogenetic analyses based on SNP dataset revealed that both serovars are closely related but showed distinct spatial clustering. However, likelihood ratio test of the indel data statistically confirmed the presence of a frameshift mutation within a homopolymeric tract of lic12008 gene (related to LPS biosynthesis) in all the L. interrogans serovar Icterohaemorrhagiae strains but not in the Copenhageni strains. Therefore, this internal indel identified can genetically distinguish L. interrogans serovar Copenhageni from serovar Icterohaemorrhagiae with high discriminatory power. To our knowledge, this is the first study to identify global sequence variations (SNPs and Indels) in L. interrogans serovars Copenhageni and Icterohaemorrhagiae.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subjectLeptospira
dc.subjectLeptospirose
dc.subjectCopenhageni
dc.subjectIcterohaemorrhagiae
dc.subjectSequenciamento do genoma completo
dc.subjectSNPs
dc.subjectIndels
dc.subjectFilogenia
dc.subjectLeptospira
dc.subjectLeptospirosis
dc.subjectCopenhageni
dc.subjectIcterohaemorrhagiae
dc.subjectWhole-genome sequencing
dc.subjectSNPs
dc.subjectIndels
dc.subjectPhylogeny
dc.titleGenomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel
dc.typeArticle


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