Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen

dc.creatorBarocchi, Michele A
dc.creatorKo, Albert Icksang
dc.creatorReis, Mitermayer Galvão dos
dc.creatorMcDonald, Kent L
dc.creatorRiley, Lee Woodland
dc.date2014-05-02T13:17:56Z
dc.date2014-05-02T13:17:56Z
dc.date2002
dc.date.accessioned2023-09-26T22:44:56Z
dc.date.available2023-09-26T22:44:56Z
dc.identifierBAROCCHI, M. A. et al. Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen. Infection and Immunity, v. 70, n. 12, p. 6926-6932, 2002.
dc.identifier0019-9567
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7566
dc.identifier10.1128/IAI.70.12.6926–6932.2002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8882710
dc.descriptionPathogenic spirochetes of the genus Leptospira are a major cause of human zoonotic infectious disease worldwide. After gaining entry through the skin, the organism causes disease by hematogenously disseminating to multiple organs. The mechanism by which it penetrates the mammalian cell barriers to disseminate is not well understood. In this study, we used a low-passage-number isolate of Leptospira interrogans to elucidate the invasive potential of this spirochete. Quantification of bacteria by dark-field microscopy revealed that pathogenic spirochetes were able to translocate through polarized MDCK cell monolayers at a rate significantly greater than that of nonpathogenic Leptospira or a recognized invasive bacterial pathogen, Salmonella. In contrast to Salmonella, L. interrogans did not alter transepithelial electrical resistance during cell translocation. Both transmission and scanning electron microscopy revealed tight association of the extracellular spirochetes with the host cell plasma membrane, without membrane perturbations suggestive of cytoskeletal rearrangement. Spirochetes were not observed within intercellular junctions or membrane-bound compartments inside cells. They were found within the cytoplasm of only 8% of the counted cells. These results indicate that Leptospira is an invasive but not a facultative intracellular organism. We propose that the rapid translocation of mammalian cells by pathogenic Leptospira is a mechanism designed to evade killing by host cells that permits the organism to quickly reach the bloodstream and disseminate to multiple organs.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectPolaridade Celular
dc.subjectCélulas Epiteliais/microbiologia
dc.subjectLeptospira interrogans/patogenicidade
dc.subjectAnimais
dc.subjectLinhagem Celular
dc.subjectCães
dc.subjectCondutividade Elétrica
dc.subjectCélulas Epiteliais/ultraestrutura
dc.subjectInterações Hospedeiro-Parasita
dc.subjectJunções Intercelulares/ultraestrutura
dc.subjectRim/citologia
dc.subjectRim/microbiologia
dc.subjectMicroscopia Eletrônica de Varredura
dc.subjectMicrovilosidades/ultraestrutura
dc.subjectFatores de Tempo
dc.subjectVirulência
dc.titleRapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen
dc.typeArticle


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