SYBR Green-based real-time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniases

dc.creatorPita-Pereira, Daniela
dc.creatorLins, Rachel
dc.creatorOliveira, Marcia P.
dc.creatorLima, Rosimar B.
dc.creatorPereira, Bernardo A. S.
dc.creatorMoreira, Otacilio C.
dc.creatorBrazil, Reginaldo P.
dc.creatorBritto, Constança
dc.date2016-08-30T11:58:42Z
dc.date2016-08-30T11:58:42Z
dc.date2012
dc.date.accessioned2023-09-26T22:44:26Z
dc.date.available2023-09-26T22:44:26Z
dc.identifierPITA-PEREIRA, Daniela; et al. SYBR Green-based Real-Time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniases. Parasites & Vectors, v.5:15, 9p, 2012.
dc.identifier1756-3305
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15514
dc.identifier10.1186/1756-3305-5-15
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8882602
dc.descriptionLeishmaniases control has been hampered by the unavailability of rapid detection methods and the lack of suitable therapeutic and prophylactic measures. Accurate diagnosis, which can distinguish between Leishmania isolates, is essential for conducting appropriate prognosis, therapy and epidemiology. Molecular methods are currently being employed to detect Leishmania infection and categorize the parasites up to genus, complex or species level. Real-time PCR offers several advantages over traditional PCR, including faster processing time, higher sensitivity and decreased contamination risk.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central
dc.rightsopen access
dc.subjectLeishmaniose
dc.subjectCurvas de dissociação térmica
dc.subjectDiagnóstico molecular
dc.subjectBrasil
dc.subjectReação em Cadeia da Polimerase
dc.subjectSYBR Green Real-time PCR
dc.subjectLeishmaniases
dc.subjectkinetoplast DNA
dc.subjectthermal dissociation curves
dc.subjectmolecular diagnosis
dc.subjectBrazil
dc.subjectDNA de Cinetoplasto
dc.titleSYBR Green-based real-time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniases
dc.typeArticle


Este ítem pertenece a la siguiente institución