Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.

dc.creatorMcBride, Alan John Alexander
dc.creatorCerqueira, Gustavo Maia de
dc.creatorSuchard, Marc A
dc.creatorMoreira, Ângela N
dc.creatorZuerner, Richard L
dc.creatorReis, Mitermayer Galvão dos
dc.creatorHaake, David A
dc.creatorKo, Albert Icksang
dc.creatorDellagostin, Odir Antonio
dc.date2014-04-25T11:49:23Z
dc.date2014-04-25T11:49:23Z
dc.date2008
dc.date.accessioned2023-09-26T22:44:20Z
dc.date.available2023-09-26T22:44:20Z
dc.identifierMcBRIDE, A. J. A. et al. Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp. Infections, Genetics and Evolution, v. 9, n. 2, p. 196-205, 2009.
dc.identifier1567-7257
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7536
dc.identifier10.1016/j.meegid.2008.10.012.
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8882584
dc.descriptionRecent serologic, immunoprotection, and pathogenesis studies identified the Lig proteins as key virulence determinants in interactions of leptospiral pathogens with the mammalian host. We examined the sequence variation and recombination patterns of ligA, ligB, and ligC among 10 pathogenic strains from five Leptospira species. All strains were found to have intact ligB genes and genetic drift accounting for most of the ligB genetic diversity observed. The ligA gene was found exclusively in L. interrogans and L. kirschneri strains, and was created from ligB by a two-step partial gene duplication process. The aminoterminal domain of LigB and the LigA paralog were essentially identical (98.5 ± 0.8% mean identity) in strains with both genes. Like ligB, ligC gene variation also followed phylogenetic patterns, suggesting an early gene duplication event. However, ligC is a pseudogene in several strains, suggesting that LigC is not essential for virulence. Two ligB genes and one ligC gene had mosaic compositions and evidence for recombination events between related Leptospira species was also found for some ligA genes. In conclusion, the results presented here indicate that Lig diversity has important ramifications for the selection of Lig polypeptides for use in diagnosis and as vaccine candidates. This sequence information will aid the identification of highly conserved regions within the Lig proteins and improve upon the performance characteristics of the Lig proteins in diagnostic assays and in subunit vaccine formulations with the potential to confer heterologous protection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherNational Institute of Health
dc.rightsopen access
dc.subjectLeptospirosis
dc.subjectLig
dc.subjectPathogenesis
dc.subjectMolecular evolution
dc.subjectSequence analysis
dc.subjectAntígenos de Bactérias/genética
dc.subjectDNA Bacteriano/genética
dc.subjectVariação Genética
dc.subjectAnimais
dc.subjectLeptospira/fisiologia
dc.subjectLeptospira/genética
dc.subjectLeptospira/imunologia
dc.subjectLeptospirose/imunologia
dc.subjectDados de Sequência Molecular
dc.subjectFilogenia
dc.subjectAnálise de Sequência de DNA
dc.subjectAnálise de Sequência de Proteína
dc.titleGenetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.
dc.typeArticle


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