The hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni

dc.creatorFrancisco, Juliane Siqueira
dc.creatorTerra, Marcia Andrea Barge Loução
dc.creatorKlein, Gabriel Couto Thurler
dc.creatorOliveira, Barbara Cristina Euzebio Pereira Dias de
dc.creatorPelajo-Machado, Marcelo
dc.date2022-10-21T17:12:07Z
dc.date2022-10-21T17:12:07Z
dc.date2022
dc.date.accessioned2023-09-26T22:40:51Z
dc.date.available2023-09-26T22:40:51Z
dc.identifierFRANCISCO, Juliane Siqueira et al. The hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni. Frontiers in Immunology, v.13:955034, p. 1 - 13, Aug. 2022.
dc.identifier1664-3224
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/55235
dc.identifier10.3389/fimmu.2022.955034
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881855
dc.descriptionMany years ago, our research group has demonstrated extramedullary hematopoiesis in the peripheral zone of murine hepatic schistosomal granulomas. In the present study, we revisit this phenomenon using new technical and conceptual approaches. Therefore, newborn mice were percutaneously infected by Schistosoma mansoni cercariae and euthanized between 35- and 60-days post infection. Liver samples were submitted to histopathology and immunohistochemical analyses. Cells under mitosis and/or expressing Ki67 demonstrated the proliferation of hematopoietic cells both around the parasite’s eggs trapped in the liver and around hepatic vessels. After 50 days post infection, proliferating cells at different levels on differentiation were located preferentially in the peripheral zone of the granulomas, around the vessels and inside the sinusoids. The presence of acidic and sulfated glycoconjugates, reticular fibers and the absence of fibronectin characterized the microenvironment for attraction and maintenance of hematopoiesis. Some neutrophils secreted MMP9 from the earliest points of infection, indicating degradation of the extracellular matrix in regions of histolysis and a possible chemoattraction of hematopoietic stem cells to the liver. Fall-3+ cells and Sca- 1+ cells indicated that early hematopoietic progenitors could be mobilized to the liver. Groups of vWF+ megakaryocytes suggest chemoattraction of these cells and/or migration, proliferation, and differentiation of very immature progenitors to this organ. The increase of blood vessels and extramedullary hematopoiesis in this environment, where markers of immature hematopoietic and endothelial cells have been identified, points to the possibility of the presence of progenitors for endothelial and hematopoietic cells in the liver during the infection. There is also the possibility of concomitant migration of more differentiated hematopoietic progenitors, that proliferate and differentiate in the liver, and the occurrence of angiogenesis caused by inflammation or release of ovular antigens that stimulate the activation and proliferation of endothelial cells. Altogether, these data increase knowledge about a murine model that is of interest for investigating the pathology of the schistosomiasis and also the dynamics of hematopoiesis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subjectEsquistossomose
dc.subjectGranuloma
dc.subjectAmbiente hematopoiético
dc.subjectAngiogênese
dc.subjectHematopoiese extramedular
dc.subjectSchistosomiasis
dc.subjectGranuloma
dc.subjectHematopoietic environment
dc.subjectAngiogenesis
dc.subjectExtramedullary hematopoiesis
dc.titleThe hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni
dc.typeArticle


Este ítem pertenece a la siguiente institución