P2X7R large pore is partially blocked by pore forming proteins antagonists in astrocytes

dc.creatorFaria, Robson X.
dc.creatorReis, Ricardo A. M.
dc.creatorFerreira, Leonardo G.B.
dc.creatorMello, Paula F. T. Cesar de
dc.creatorMoraes, Milton O.
dc.date2016-12-27T10:40:28Z
dc.date2016-12-27T10:40:28Z
dc.date2016
dc.date.accessioned2023-09-26T22:40:40Z
dc.date.available2023-09-26T22:40:40Z
dc.identifierFARIA, Robson X. et al. P2X7R large pore is partially blocked by pore forming proteins antagonists in astrocytes. J Bioenerg Biomembr, v.48, p.309–324, 2016.
dc.identifier0145-479X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16568
dc.identifier10.1007/s10863-016-9649-9
dc.identifier1573-6881
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881817
dc.descriptionThe ATP-gated P2X7R (P2X7R) is a channel, which is involved in events, such as inflammation, cell death, and pain. The most intriguing event concerning P2X7R functions is the phenomenon of pore dilation. Once P2X7R is activated, the permeability of the plasma membrane becomes higher, leading to the permeation of 1000 Da-weight solutes. The mechanisms involved in this process remain unclear. Nevertheless, this event is not exclusively through P2X7R, as other proteins may form large pores in the plasma membrane. Recent evidence concerning pore formation reveals putative P2X7R and other pores-associated protein complexes, revealing cross-interactive pharmacological and biophysical issues. In this work, we showed results that corroborated with cross-interactive aspects with P2X7R and pores in astrocytes. These cells expressed most of the pores, including P2X7R. We discovered that different pore types open with peculiar characteristics, as both anionic and cationic charged solutes permeate the plasma membrane, following P2X7R activation. Moreover, we showed that both synergic and additive relationships are found within P2X7, cationic, and anionic large pores. Therefore, our data suggest that other protein-related pores are assembled following the formation of P2X7R pore.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Verlag
dc.rightsrestricted access
dc.subjectEletrofisiologia
dc.subjectAntagonistas de Hemichannel
dc.subjectFormação de poros
dc.subjectHemichannels
dc.subjectP2X7R
dc.subjectPore formation
dc.subjectHemichannels
dc.subjectHemichannel antagonists
dc.subjectElectrophysiology
dc.titleP2X7R large pore is partially blocked by pore forming proteins antagonists in astrocytes
dc.typeArticle


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