Implications of the use of serological and molecular methods to detect infection by Leishmania spp. in urban pet dogs.

dc.creatorFontes, Paz Gustavo
dc.creatorRugani, Jeronimo Marteleto Nunes
dc.creatorMarcelino, Andreza Pain
dc.creatorFerreira, Gontijo Celia Maria
dc.date2018-09-18T13:49:05Z
dc.date2018-09-18T13:49:05Z
dc.date2018
dc.date.accessioned2023-09-26T22:40:09Z
dc.date.available2023-09-26T22:40:09Z
dc.identifierPAZ, Gustavo Fontes et al. Implications of the use of serological and molecular methods to detect infection by Leishmania spp. in urban pet dogs. Acta Trop., v. 182, p. 198-201, 2018.
dc.identifier0001-706X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28857
dc.identifier10.1016/j.actatropica.2018.03.018
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881711
dc.descriptionThe aim of this study was to evaluate the relationship between naturally occurring Leishmania spp. infections in dogs (Canis familiaris) and the practical implications of the use of serological and molecular methods to confirm diagnoses. The study population consisted of 96 domestic dogs in southeastern Brazil. Serum samples were tested for the presence of anti-Leishmania immunoglobulin G (IgG) antibodies using four commercial canine visceral leishmaniasis kits. Dogs confirmed positive by immunofluorescence antibody test (IFAT) were culled and samples from mesenteric lymph nodes, spleen border, bone marrow and ear skin were taken and submitted to DNA extraction. PCR reactions were performed using primers that amplify a 300–350 bp fragment of the Leishmania ribosomal internal transcribed spacer 1 (ITS1) region. The ITS1 amplified products were analyzed by PCR-RFLP using Hae III restriction endonuclease. To confirm the Leishmania species detected by PCR, each purified sample was sequenced in duplicate. Of the 96 serum samples submitted to serological assays, 8 (8.3%) tested positive for Leishmania by IFAT, 4 (4.1%) by ELISA, 2 (2.1%) by rK39 RDT and 7 (7.3%) by DPP. Four of these infected dogs (50%) were found to be infected only by Leishmania braziliensis or Leishmania amazonensis, and their serum samples tested positive by IFAT and DPP. These findings demonstrate for the first time that cross-reactivity of L. braziliensis and L. amazonensis infection in dogs can be found using the DPP serum test. This is the first record of Leishmania (Leishmania) amazonensis confirmed by a specific molecular marker in dogs (Canis familiaris) from Belo Horizonte, Brazil.
dc.description2024-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectLeishmania braziliensis
dc.subjectLeishmania amazonensis
dc.subjectLeishmania infantum
dc.subjectcachorros
dc.subjectensaios sorologicos
dc.subjectLeishmania braziliensis
dc.subjectLeishmania amazonensis
dc.subjectLeishmania infantum
dc.subjectDogs
dc.subjectSerological assays
dc.titleImplications of the use of serological and molecular methods to detect infection by Leishmania spp. in urban pet dogs.
dc.typeArticle


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