Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review

dc.creatorCunha, Mylena Lemes
dc.creatorSilva, Stella Schuster da
dc.creatorStracke, Mateus Cassaboni
dc.creatorZanette, Dalila Luciola
dc.creatorAoki, Mateus Nóbrega
dc.creatorBlanes, Lucas
dc.date2022-03-24T14:58:45Z
dc.date2022-03-24T14:58:45Z
dc.date2022
dc.date.accessioned2023-09-26T22:39:57Z
dc.date.available2023-09-26T22:39:57Z
dc.identifierCUNHA, Mylena Lemes et al. Sample Preparation for Lab-on-a-Chip Systems in Molecular Diagnosis: A Review. Anal. Chem., n. 94, p. 41−58, 2022.
dc.identifier1520-6882
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/51855
dc.identifier10.1021/acs.analchem.1c04460
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881676
dc.descriptionRapid and low-cost molecular analysis is especially required for early and specific diagnostics, quick decision-making, and sparing patients from unnecessary tests and hospitals from extra costs. One way to achieve this objective is through automated molecular diagnostic devices. Thus, sample-to-answer microfluidic devices are emerging with the promise of delivering a complete molecular diagnosis system that includes nucleic acid extraction, amplification, and detection steps in a single device. The biggest issue in such equipment is the extraction process, which is normally laborious and time-consuming but extremely important for sensitive and specific detection. Therefore, this Review focuses on automated or semiautomated extraction methodologies used in lab-on-a-chip devices. More than 15 different extraction methods developed over the past 10 years have been analyzed in terms of their advantages and disadvantages to improve extraction procedures in future studies. Herein, we are able to explain the high applicability of the extraction methodologies due to the large variety of samples in which different techniques were employed, showing that their applications are not limited to medical diagnosis. Moreover, we are able to conclude that further research in the field would be beneficial because the methodologies presented can be affordable, portable, time efficient, and easily manipulated, all of which are strong qualities for point-of-care technologies.
dc.formatapplication/pdf
dc.languagepor
dc.publisherAmerican Chemical Society
dc.rightsopen access
dc.subjectMolecular Diagnostic Testing
dc.subjectLab-On-A-Chip Devices
dc.subjectAnalytic Sample Preparation Methods
dc.subjectPoint-of-Care Systems
dc.subjectTécnicas de Diagnóstico Molecular
dc.subjectDispositivos Laboratorio en un Chip
dc.subjectMétodos Analíticos de la Preparación de la Muestra
dc.subjectSistemas de Atención de Punto
dc.subjectTechniques de diagnostic moléculaire
dc.subjectLaboratoires sur puces
dc.subjectMéthodes de préparation d'échantillons analytiques
dc.subjectSystèmes automatisés lit malade
dc.subjectTeste de Diagnóstico Molecular
dc.subjectDispositivos Analíticos em Microchips
dc.subjectMétodos Analíticos de Preparação de Amostras
dc.subjectSistemas Automatizados de Assistência Junto ao Leito
dc.titleSample preparation for lab-on-a-chip systems in molecular diagnosis: a review
dc.typeArticle


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