Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization

dc.creatorBusatti, Haendel Gonçalves Nogueira Oliveira
dc.creatorAlves, Ricardo José
dc.creatorAnjos, Karla Graziela Santana dos
dc.creatorGil, Frederico Ferreira
dc.creatorCury, Marcia Cristina
dc.creatorSantos, Marcos André Vannier dos
dc.creatorGomes, Maria Aparecida
dc.date2017-08-25T17:49:57Z
dc.date2017-08-25T17:49:57Z
dc.date2013
dc.date.accessioned2023-09-26T22:39:26Z
dc.date.available2023-09-26T22:39:26Z
dc.identifierBUSATTI, H. G. N. O. et al. Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization. Diagnostic Microbiology and Infectious Disease, v. 75, p. 160-164, 2013.
dc.identifier0732-8893
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20773
dc.identifier10.1016/j.diagmicrobio.2012.11.001
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881563
dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas–FAPEMIG (grant number APQ 01766–10), FAPESB, PP-SUS, CNPq, and FIOCRUZ
dc.descriptionThe chemotherapeutic agents used for the treatment of giardiasis are often associated with adverse side effects and are refractory cases, due to the development of resistant parasites. Therefore the search for new drugs is required. We have previously reported the giardicidal effects of metronidazole (MTZ) and its analogues (MTZ-Ms, MTZ-Br, MTZ-N(3), and MTZ-I) on the trophozoites of Giardia lamblia. Now we evaluated the activity of some giardicidal MTZ analogues in experimental infections in gerbils and its effects on the morphology and ultrastructural organization of Giardia. The giardicidal activity in experimental infections showed ED(50) values significantly lower for MTZ-I and MTZ-Br when compared to MTZ. Transmission electron microscopy was employed to approach the mechanism(s) of action of MTZ analogues upon the protozoan. MTZ analogues were more active than MTZ in changing significantly the morphology and ultrastructure of the parasite. The analogues affected parasite cell vesicle trafficking, autophagy, and triggered differentiation into cysts. These results coupled with the excellent giardicidal activity and lower toxicity demonstrate that these nitroimidazole derivates may be important therapeutic alternatives for combating giardiasis. In addition, our results suggest a therapeutic advantage in obtaining synthetic metronidazole analogues for screening of activities against other infectious agents.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectGiardia Lamblia
dc.subjectMetais análogos de metronidazol
dc.subjectCarga parasitária
dc.subjectMicroscopia eletrônica
dc.subjectGiardicidal activity
dc.subjectMetronidazole analogues
dc.subjectParasite load
dc.subjectElectron microscopy
dc.titleEffects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization
dc.typeArticle


Este ítem pertenece a la siguiente institución