Promising approach to reducing Malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi

dc.creatorPinilla, Yudi Tatiana
dc.creatorLopes, Stefanie Costa Pinto
dc.creatorSampaio, Vanderson Souza
dc.creatorAndrade, Francys S.
dc.creatorMelo, Gisely Cardoso de
dc.creatorOrfanó, Alessandra da Silva
dc.creatorSecundino, Nagila Francinete Costa
dc.creatorGuerra, Maria das Graças Vale Barbosa
dc.creatorLacerda, Marcus Vinícius Guimarães de
dc.creatorKobylinski, Kevin C.
dc.creatorEscobedo-Vargas, Karin S.
dc.creatorLopez-Sifuentes, Victor M.
dc.creatorStoops, Craig A.
dc.creatorBaldeviano, G. Christian
dc.creatorTarning, Joel
dc.creatorVasquez, Gissella M.
dc.creatorPimenta, Paulo Filemon Paolucci
dc.creatorMonteiro, Wuelton Marcelo
dc.date2018-09-13T18:45:45Z
dc.date2018-09-13T18:45:45Z
dc.date2018
dc.date.accessioned2023-09-26T22:38:33Z
dc.date.available2023-09-26T22:38:33Z
dc.identifierPINILLA, Yudi Tatiana et al. Promising approach to reducing Malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi. PLoS Negl Trop Dis., v. 12, n. 2, e0006221, 2018
dc.identifier1935-2735
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28771
dc.identifier10.1371/journal.pntd.0006221
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881386
dc.descriptionBACKGROUND: The mosquito resistance to the insecticides threatens malaria control efforts, potentially becoming a major public health issue. Alternative methods like ivermectin (IVM) administration to humans has been suggested as a possible vector control to reduce Plasmodium transmission. Anopheles aquasalis and Anopheles darlingi are competent vectors for Plasmodium vivax, and they have been responsible for various malaria outbreaks in the coast of Brazil and the Amazon Region of South America. METHODS: To determine the IVM susceptibility against P. vivax in An. aquasalis and An. darlingi, ivermectin were mixed in P. vivax infected blood: (1) Powdered IVM at four concentrations (0, 5, 10, 20 or 40 ng/mL). (2) Plasma (0 hours, 4 hours, 1 day, 5, 10 and 14 days) was collected from healthy volunteers after to administer a single oral dose of IVM (200 μg/kg) (3) Mosquitoes infected with P. vivax and after 4 days was provided with IVM plasma collected 4 hours post-treatment (4) P. vivax-infected patients were treated with various combinations of IVM, chloroquine, and primaquine and plasma or whole blood was collected at 4 hours. Seven days after the infective blood meal, mosquitoes were dissected to evaluate oocyst presence. Additionally, the ex vivo effects of IVM against asexual blood-stage P. vivax was evaluated. RESULTS: IVM significantly reduced the prevalence of An. aquasalis that developed oocysts in 10 to 40 ng/mL pIVM concentrations and plasma 4 hours, 1 day and 5 days. In An. darlingi to 4 hours and 1 day. The An. aquasalis mortality was expressively increased in pIVM (40ng/mL) and plasma 4 hours, 1, 5 10 and 14 days post-intake drug and in An. darlingi only to 4 hours and 1 day. The double fed meal with mIVM by the mosquitoes has a considerable impact on the proportion of infected mosquitoes for 7 days post-feeding. The oocyst infection prevalence and intensity were notably reduced when mosquitoes ingested blood from P. vivax patients that ingested IVM+CQ, PQ+CQ and IVM+PQ+CQ. P. vivax asexual development was considerably inhibited by mIVM at four-fold dilutions. CONCLUSION: In conclusion, whole blood spiked with IVM reduced the infection rate of P. vivax in An. aquasalis and An. darlingi, and increased the mortality of mosquitoes. Plasma from healthy volunteers after IVM administration affect asexual P. vivax development. These findings support that ivermectin may be used to decrease P. vivax transmission.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectMalaria
dc.subjectP. vivax
dc.subjectMalaria
dc.subjectP. vivax
dc.titlePromising approach to reducing Malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi
dc.typeArticle


Este ítem pertenece a la siguiente institución