Schwann Cells Producing Matrix Metalloproteinases Under Mycobacterium leprae Stimulation May Play a Role in the Outcome of Leprous Neuropathy

dc.creatorOliveira, Ariane Leite
dc.creatorAntunes, Sérgio Luiz Gomes
dc.creatorTeles, Rosane Magda
dc.creatorSilva, Ana Carolina Costa da
dc.creatorSilva, Tatiana Pereira da
dc.creatorTeles, Rose Brandão
dc.creatorMedeiros, Mildred Ferreira
dc.creatorBritto, Constança
dc.creatorJardim, Márcia Rodrigues
dc.creatorSampaio, Elizabeth Pereira
dc.creatorSarno, Euzenir Nunes
dc.date2017-09-14T18:31:33Z
dc.date2017-09-14T18:31:33Z
dc.date2010
dc.date.accessioned2023-09-26T22:38:28Z
dc.date.available2023-09-26T22:38:28Z
dc.identifierOLIVEIRA, Ariane Leite; et al. Schwann Cells Producing Matrix Metalloproteinases Under Mycobacterium leprae Stimulation May Play a Role in the Outcome of Leprous Neuropathy. J. Neuropathol Exp Neurol., v.69, n.1, p.27-39, Jan. 2010.
dc.identifier0022-3069
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/21011
dc.identifier1554-6578
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881370
dc.descriptionMatrix metalloproteinases (MMPs) mediate demyelination and breakdown of the blood-nerve barrier in peripheral neuropathies. Matrix metalloproteinases and tissue inhibitor of metalloproteinase 1 gene expression and secretion were studied in cells of the human Schwann cell line ST88-14 stimulated with Mycobacterium leprae and tumor necrosis factor (TNF) and in nerve biopsies from patients with neural leprosy (n = 21) and nonleprous controls (n = 3). Mycobacterium leprae and TNF induced upregulation of MMP-2 and MMP-9 and increased secretion of these enzymes in cultured ST88-14 cells. The effects of TNF and M. leprae were synergistic, and anti-TNF antibody blockage partially inhibited this synergistic effect. Nerves with inflammatory infiltrates and fibrosis displayed higher TNF, MMP-2, and MMP-9 mRNA than controls. Leprous nerve biopsies with no inflammatory alterations also exhibited higher MMP-2 and MMP-9; tissue inhibitor of metalloproteinase 1 was significantly higher in biopsies with fibrosis and inflammation. Immunohistochemical double labeling of the nerves demonstrated that the MMPs were mainly expressed by macrophages and Schwann cells. The biopsies with endoneurial inflammatory infiltrates and epithelioid granulomas had the highest levels of MMP-2 and MMP-9 mRNA detected. Together, these results suggest that M. leprae and TNF may directly induce Schwann cells to upregulate and secrete MMPs regardless of the extent of inflammation in leprous neuropathy.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherOxford University Press
dc.rightsrestricted access
dc.subjectFibrose
dc.subjectHanseníase
dc.subjectMetaloproteinases da Matriz
dc.subjectDoenças do Sistema Nervoso Periférico
dc.subjectFator de Necrose Tumoral alfa
dc.subjectFibrosis
dc.subjectLeprosy
dc.subjectMatrix metalloproteinases
dc.subjectPeripheral neuropathy
dc.subjectTumor necrosis factor
dc.titleSchwann Cells Producing Matrix Metalloproteinases Under Mycobacterium leprae Stimulation May Play a Role in the Outcome of Leprous Neuropathy
dc.typeArticle


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