Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp

dc.creatorGuimarães, Elisalva Teixeira
dc.creatorCruz, Gabriela da Silva
dc.creatorJesus, Alan Araújo de
dc.creatorCarvalho, Acácia Fernandes Lacerda de
dc.creatorRogatto, Silvia Regina
dc.creatorPereira, Lygia da Veiga
dc.creatorSantos, Ricardo Ribeiro dos
dc.creatorSoares, Milena Botelho Pereira
dc.date2014-02-18T13:02:23Z
dc.date2014-02-18T13:02:23Z
dc.date2011
dc.date.accessioned2023-09-26T22:38:18Z
dc.date.available2023-09-26T22:38:18Z
dc.identifierGUIMARÃES, E. T. et al. Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp. Archives of Oral Biology, v. 56, n. 11, p. 1247-1255, 2011.
dc.identifier1879-1506
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7318
dc.identifier10.1016/j.archoralbio.2011.05.008
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881334
dc.descriptionObjective: Several studies have demonstrated that human dental pulp is a source of mesenchymal stem cells. To better understand the biological properties of these cells we isolated and characterized stem cells from the dental pulp of EGFP transgenic mice. Methods: The pulp tissue was gently separated from the roots of teeth extracted from C57BL/ 6 mice, and cultured under appropriate conditions. Flow cytometry, RT-PCR, light microscopy (staining for alkaline phosphatase) and immunofluorescence were used to investigate the expression of stem cell markers. The presence of chromosomal abnormalities was evaluated by G banding. Results: The mouse dental pulp stem cells (mDPSC) were highly proliferative, plasticadherent, and exhibited a polymorphic morphology predominantly with stellate or fusiform shapes. The presence of cell clusters was observed in cultures of mDPSC. Some cells were positive for alkaline phosphatase. The karyotype was normal until the 5th passage. The Pou5f1/Oct-4 and ZFP42/Rex-1, but not Nanog transcripts were detected in mDPSC. Flow cytometry and fluorescence analyses revealed the presence of a heterogeneous population positive for embryonic and mesenchymal cell markers. Adipogenic, chondrogenic and osteogenic differentiation was achieved after two weeks of cell culture under chemically defined in vitro conditions. In addition, some elongated cells spontaneously acquired a contraction capacity. Conclusions: Our results reinforce that the dental pulp is an important source of adult stem cells and encourage studies on therapeutic potential of mDPSC in experimental disease models
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectDental pulp
dc.subjectStem cells
dc.subjectMouse
dc.subjectPhenotype
dc.subjectDifferentiation
dc.subjectCélulas-Tronco Adultas/citologia
dc.subjectPolpa Dentária/citologia
dc.subjectPolpa Dentária/embriologia
dc.subjectCélulas-Tronco Embrionárias/citologia
dc.subjectCélulas Mesenquimais Estromais/citologia
dc.subjectAdipogenia
dc.subjectAnimais
dc.subjectDiferenciação Celular
dc.subjectCondrogênese
dc.subjectProteínas de Ligação a DNA/biossíntese
dc.subjectProteínas de Ligação a DNA/genética
dc.subjectCitometria de Fluxo
dc.subjectMarcadores Genéticos
dc.subjectCariótipo
dc.subjectCamundongos
dc.subjectCamundongos Endogâmicos C57BL
dc.subjectCamundongos Transgênicos
dc.subjectFator 3 de Transcrição de Octâmero/biossíntese
dc.subjectFator 3 de Transcrição de Octâmero/genética
dc.subjectOsteogênese
dc.subjectFenótipo
dc.subjectFatores de Transcrição/biossíntese
dc.subjectFatores de Transcrição/genética
dc.titleMesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
dc.typeArticle


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