Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis

dc.creatorNascimento, Ana Paula Barbosa do
dc.creatorMedeiros Filho, Fernando
dc.creatorPauer, Heidi
dc.creatorAntunes, Luis Caetano Martha
dc.creatorSousa, Hério
dc.creatorSenger, Hermes
dc.creatorAlbano, Rodolpho Mattos
dc.creatorSantos, Marcelo Trindade dos
dc.creatorCarvalho-Assef, Ana Paula D’Alincourt
dc.creatorSilva, Fabrício Alves Barbosa da
dc.date2021-02-11T19:05:10Z
dc.date2021-02-11T19:05:10Z
dc.date2020
dc.date.accessioned2023-09-26T22:37:28Z
dc.date.available2023-09-26T22:37:28Z
dc.identifierNASCIMENTO, Ana Paula Barbosa do et al. Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis. Scientific Reports, v. 20, 13192, 16p, 2020.
dc.identifier2045-2322
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/46046
dc.identifier10.1038/s41598-020-69944-6
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881156
dc.descriptionPseudomonas aeruginosa is one of the most common pathogens related to healthcare-associated infections. The Brazilian isolate, named CCBH4851, is a multidrug-resistant clone belonging to the sequence type 277. The antimicrobial resistance mechanisms of the CCBH4851 strain are associated with the presence of the blaSPM-1 gene, encoding a metallo-beta-lactamase, in combination with other exogenously acquired genes. Whole-genome sequencing studies focusing on emerging pathogens are essential to identify key features of their physiology that may lead to the identification of new targets for therapy. Using both Illumina and PacBio sequencing data, we obtained a single contig representing the CCBH4851 genome with annotated features that were consistent with data reported for the species. However, comparative analysis with other Pseudomonas aeruginosa strains revealed genomic differences regarding virulence factors and regulatory proteins. In addition, we performed phenotypic assays that revealed CCBH4851 is impaired in bacterial motilities and biofilm formation. On the other hand, CCBH4851 genome contained acquired genomic islands that carry transcriptional factors, virulence and antimicrobial resistance-related genes. Presence of single nucleotide polymorphisms in the core genome, mainly those located in resistance-associated genes, suggests that these mutations may also influence the multidrug-resistant behavior of CCBH4851. Overall, characterization of Pseudomonas aeruginosa CCBH4851 complete genome revealed the presence of features that strongly relates to the virulence and antibiotic resistance profile of this important infectious agent.
dc.formatapplication/pdf
dc.languageeng
dc.publisherNature Research
dc.rightsopen access
dc.subjectPseudomonas aeruginosa
dc.subjectSPM-1
dc.subjectProdutoras de metalo-beta-lactamase
dc.subjectGenômica comparativa
dc.subjectAnálise fenotípica
dc.subjectSPM‑1
dc.subjectMetallo‑beta‑lactamase‑producing
dc.subjectPseudomonas aeruginosa
dc.subjectComparative genomics
dc.subjectPhenotypic analysis
dc.titleCharacterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis
dc.typeArticle


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