Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK

dc.creatorBottrel, R. L
dc.creatorDutra, Walderez Ornelas
dc.creatorMartins, Fabrícia Andrade
dc.creatorGontijo, B
dc.creatorCarvalho Filho, Edgar Marcelino
dc.creatorBarral Netto, Manoel
dc.creatorBarral, Aldina Maria Prado
dc.creatorAlmeida, Roque Pacheco de
dc.creatorMayrink, Wilson
dc.creatorLocksley, R
dc.creatorGollob, Kenneth J
dc.date2014-03-25T12:59:44Z
dc.date2014-03-25T12:59:44Z
dc.date2001
dc.date.accessioned2023-09-26T22:37:13Z
dc.date.available2023-09-26T22:37:13Z
dc.identifierBOTTREL, R. L. et al. Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK. Infection and Immunity, v. 69, n. 5, p.3232-3239, 2001.
dc.identifier0019-9567
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7442
dc.identifier10.1128/IAI.69.5.3232–3239.2001
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881102
dc.descriptionLeishmaniasis, caused by infection with the protozoan parasite Leishmania, affects millions of individuals worldwide, causing serious morbidity and mortality. This study directly determined the frequency of cells producing key immunoregulatory cytokines in response to the recombinant antigen Leishmania homolog of receptors for activated kinase C (LACK) and soluble leishmania antigen (SLA), and it determined relative contributions of these antigens to the overall cytokine profile in individuals infected for the first time with Leishmania braziliensis. All individuals presented with the cutaneous clinical form of leishmaniasis and were analyzed for proliferative responses to LACK antigen and SLA, frequency of lymphocyte subpopulations (analyzed ex vivo), and antigen-induced (LACK and SLA) cytokine production at the single-cell level (determined by flow cytometry). The following were determined. (i) The Th1-type response previously seen in patients with cutaneous leishmaniasis is due to gamma interferon (IFN-g) production by several different sources, listed in order of contribution: CD41 T lymphocytes, CD42, CD82 lymphocytes, and CD81 T lymphocytes. (ii) SLA induced a higher frequency of lymphocytes producing IFN-g and tumor necrosis factor alpha (TNF-a) than did LACK. (iii) LACK induced an activation of monocyte populations as reflected by an increased percentage of CD14-positive cells. (iv) Neither SLA nor LACK induced detectable frequencies of cells producing interleukin-4 (IL-4) or IL-5. These data demonstrated a multifaceted immune response to SLA in human leishmaniasis involving Th1 CD41 T lymphocytes (IFN-g1 and IL-102/IL-42), Tc1 CD81 T cells (IFN-g1, and IL-102/IL-42), and a high frequency of TNF-a-producing lymphocytes. Moreover, it was determined that the recombinant antigen LACK acts as a weak inducer of Th1-type lymphocyte responses compared to SLA.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectAntígenos de Protozoários/imunologia
dc.subjectCitocinas/biossíntese
dc.subjectCitometria de Fluxo
dc.subjectLeishmaniose Cutânea/imunologia
dc.subjectProteínas de Protozoários/imunologia
dc.subjectHumanos
dc.subjectCélulas Th1/imunologia
dc.subjectInterferon gama/biossíntese
dc.subjectInterleucina-10/biossíntese
dc.subjectProteínas Recombinantes/imunologia
dc.subjectFator de Necrose Tumoral alfa/biossíntese
dc.titleFlow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK
dc.typeArticle


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