Chromosome damage induced by 5-azacytidine under the influence of caffeine or cytosine arabinoside in CHO-K1 (wild-type) and XRS-5 (mutant) cell lines

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSakamoto-Hojo, E. T.
dc.creatorBicego-Nahas, K. C.
dc.creatorTakahashi-Hyodo, S. A.
dc.creatorTavares, D. C.
dc.creatorDias, F. L.
dc.date2014-05-27T11:20:17Z
dc.date2016-10-25T18:17:07Z
dc.date2014-05-27T11:20:17Z
dc.date2016-10-25T18:17:07Z
dc.date2001-07-14
dc.date.accessioned2017-04-06T00:59:50Z
dc.date.available2017-04-06T00:59:50Z
dc.identifierCancer Research, Therapy and Control, v. 11, n. 2, p. 125-134, 2001.
dc.identifier1064-0525
dc.identifierhttp://hdl.handle.net/11449/66554
dc.identifierhttp://acervodigital.unesp.br/handle/11449/66554
dc.identifier2-s2.0-0034959715
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/888106
dc.description5-azacytidine (5-azaC) treatment combined with cytosine arabinoside (ara-C) or caffeine were performed in vitro in Chinese hamster cells, CHO-K1 (wild-type) and xrs-5 (mutant) cell lines, in order to compare the cell response to the induction of chromosomal aberrations. Exponentially growing cells were treated with 5-azaC (4-16 uM) for 1 h, the cells were washed and incubated for 7 h, and 500 uM caffeine or 5 uM ara-C were added to the cultures for the last 2 h. In both cell lines, 5-azaC induced a significantly increase (P<0.01) in the frequencies of aberrations; in the combined treatments (5-azaC + Ara-C), a significant reduction (P<0.05) was observed for the aberrations which were randomly distributed. Caffeine had no influence at the same conditions. 5-azaC induced-DNA lesions were probably processed at S/G2 phase in a common pathway in both cell lines, but alternatively, 5-azaC may cause xrs-5 cells to revert to the wild-type.
dc.languageeng
dc.relationCancer Research, Therapy and Control
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject5-Azacytidine
dc.subjectCaffeine
dc.subjectChromosomal aberrations
dc.subjectCytosine arabionside
dc.subjectazacitidine
dc.subjectcaffeine
dc.subjectcytarabine
dc.subjectanimal cell
dc.subjectcell cycle G2 phase
dc.subjectcell cycle S phase
dc.subjectcell growth
dc.subjectcell line
dc.subjectCHO cell
dc.subjectchromosome aberration
dc.subjectchromosome damage
dc.subjectcontrolled study
dc.subjectnonhuman
dc.subjectpriority journal
dc.titleChromosome damage induced by 5-azacytidine under the influence of caffeine or cytosine arabinoside in CHO-K1 (wild-type) and XRS-5 (mutant) cell lines
dc.typeOtro


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