A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs

dc.creatorOrjuela-Sánchez, Pamela
dc.creatorDuggan, Erika
dc.creatorNolan, John
dc.creatorFrangos, John A.
dc.creatorCarvalho, Leonardo J. M.
dc.date2017-04-18T16:07:33Z
dc.date2017-04-18T16:07:33Z
dc.date2012
dc.date.accessioned2023-09-26T22:36:23Z
dc.date.available2023-09-26T22:36:23Z
dc.identifierORJUELA-SANCHEZ, Pamela; et al. A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs. Malaria Journal, v.11:366, 8p, Nov. 2012.
dc.identifier1475-287
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/18497
dc.identifier10.1186/1475-2875-11-366
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8880921
dc.descriptionPlasmodium berghei rodent malaria is a well-known model for the investigation of anti-malarial drug efficacy in vivo. However, the availability of drug in vitro assays in P. berghei is reduced when compared with the spectrum of techniques existing for Plasmodium falciparum. New alternatives to the current manual or automated methods described for P. berghei are attractive. The present study reports a new ELISA drug in vitro assay for P. berghei using two monoclonal antibodies against the parasite lactate dehydrogenase (pLDH).
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central
dc.rightsopen access
dc.subjectPlasmodium berghei
dc.subjectCultura in vitro
dc.subjectInfecção sincronizada
dc.subjectEnsaio in vitro com fármaco
dc.subjectVacinas Antimaláricas
dc.subjectL-Lactato Desidrogenase
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectIn vitro culture
dc.subjectschizont
dc.subjectsynchronized infection
dc.subjectdrug in vitro assay
dc.subjectanti-malarials
dc.subjectIC50 values
dc.subjectlactate dehydrogenase
dc.subjectELISA
dc.subjectPlasmodium berghei
dc.titleA lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs
dc.typeArticle


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