Rolling-circle amplification of Torque teno virus (TTV) complete genomes from human and swine sera and identification of a novel swine TTV genogroup

dc.creatorNiel, Christian
dc.creatorMendes, Leonardo Diniz
dc.creatorDevalle, Sylvie
dc.date2019-12-12T16:32:42Z
dc.date2019-12-12T16:32:42Z
dc.date2005
dc.date.accessioned2023-09-26T22:36:04Z
dc.date.available2023-09-26T22:36:04Z
dc.identifierNIEL, Christian; MENDES, Leonardo Diniz; DEVALLE, Sylvie. Rolling-circle amplification of Torque teno virus (TTV) complete genomes from human and swine sera and identification of a novel swine TTV genogroup. Journal of General Virology, v. 86, p. 1343-1347, 2005.
dc.identifier0022-1317
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/37914
dc.identifier10.1099/vir.0.80794-0
dc.identifier1465-2099
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8880863
dc.descriptionMultiply primed rolling-circle amplification is a novel technology that uses bacteriophage phi29 DNA polymerase to amplify circular DNA molecules, without the need for prior knowledge of their sequences. In an attempt to detect Torque teno virus (TTV), rolling-circle amplification was used to amplify DNA extracted from eight human and four pig serum samples. All samples gave high molecular weight (>30 kb) amplification products. By restriction endonuclease digestion, these products generated DNA fragments whose sizes were consistent with those of human TTV (3?8 kb) and swine TTV (Sd-TTV; 2?9 kb) genomes. Two TTV isolates derived from a single AIDS patient, as well as two Sd-TTV isolates derived from a single pig, were characterized by complete nucleotide sequencing. One of the Sd-TTV isolates showed very low (43–45 %) nucleotide sequence similarity to the other Sd-TTV isolate and to the prototype isolate Sd-TTV31, and could be considered the prototype of a novel genogroup.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherMicrobiology Society
dc.rightsrestricted access
dc.subjectTorque teno virus
dc.subjectGenoma Completo
dc.subjectHumanos
dc.subjectSuínos
dc.subjectSoros
dc.subjectIdentificação
dc.subjectTorque teno virus
dc.subjectComplete genomes
dc.subjectHuman
dc.subjectSwine
dc.subjectSera
dc.subjectIdentification
dc.titleRolling-circle amplification of Torque teno virus (TTV) complete genomes from human and swine sera and identification of a novel swine TTV genogroup
dc.typeArticle


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