L-amino acid oxidase activity present in fractions of Bothrops jararaca venom is responsible for the induction of programmed cell death in Trypanosoma cruzi

dc.creatorDeolindo, Poliana
dc.creatorFerreira, André S. Teixeira
dc.creatorDaMatta, Renato A.
dc.creatorAlves, Elias W.
dc.date2017-09-12T17:10:55Z
dc.date2017-09-12T17:10:55Z
dc.date2010
dc.date.accessioned2023-09-26T22:35:29Z
dc.date.available2023-09-26T22:35:29Z
dc.identifierDEOLINDO, Poliana; et al. L-Amino acid oxidase activity present in fractions of Bothrops jararaca venom is responsible for the induction of programmed cell death in Trypanosoma cruzi. Toxicon, v.56, p.944-955, 2010.
dc.identifier0041-0101
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20977
dc.identifier10.1016/j.toxicon.2010.06.019
dc.identifier1879-3150
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8880753
dc.descriptionBothrops jararaca venom induces programmed cell death in epimastigotes of Trypanosoma cruzi. Here we fractionated the venom and observed that the anti-T. cruzi activity was associated with fractions that present L-amino acid oxidase (L-AAO) activity. L-AAO produces H(2)O(2), which is highly toxic. The addition of catalase to the medium, a H(2)O(2) scavenger, reverted the killing capacity of venom fractions. The anti-T. cruzi activity was also abolished when parasites were cultured in a medium without hydrophobic amino acids that are essential for L-AAO activity. These results were confirmed with a commercial purified L-AAO. Treatment for 24 h with fractions that present L-AAO activity induced parasites cytoplasmic retraction, mitochondrial swelling and DNA fragmentation, all morphological characteristics of programmed cell death. Similar changes were also observed when parasites were treated with H(2)O(2). These results indicate that H(2)O(2), the product of L-AAO reaction, induces programmed cell death explaining the anti-T. cruzi activity of B. jararaca venom.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectMorte celular programada
dc.subjectVeneno de jararaca
dc.subjectL-Aminoácido Oxidase
dc.subjectBothrops jararaca venom
dc.subjectL-amino acid oxidase
dc.subjectProgrammed cell death
dc.subjectTrypanosoma cruzi
dc.titleL-amino acid oxidase activity present in fractions of Bothrops jararaca venom is responsible for the induction of programmed cell death in Trypanosoma cruzi
dc.typeArticle


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