Antibody responses induced by Leish-Tec®, an A2-based vaccine for visceral leishmaniasis, in a heterogeneous canine population Miriam

dc.creatorTestasicca, Miriam C. de Souza
dc.creatorSantos, Mariana Silva dos
dc.creatorMachado, Leopoldo Marques
dc.creatorSerufo, Angela Vieira
dc.creatorDoro, Daniel
dc.creatorAvelar, Daniel de
dc.creatorTibúrcio, Ana Maria Leonardi
dc.creatorAbrantes, Christiane de Freitas
dc.creatorCoelho, George Luiz Lins Machado
dc.creatorGrimaldi Junior, Gabriel
dc.creatorGazzinelli, Ricardo Tostes
dc.creatorFernandes, Ana Paula
dc.date2014-06-04T18:41:08Z
dc.date2014-06-04T18:41:08Z
dc.date2014
dc.date.accessioned2023-09-26T22:34:54Z
dc.date.available2023-09-26T22:34:54Z
dc.identifierTESTASICCA, M. C. de S. et al. Antibody responses induced by Leish-Tec®, an A2-based vaccine for visceral leishmaniasis, in a heterogeneous canine population Miriam. Veterinary Parasitology, 2014.
dc.identifier0304-4017
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7793
dc.identifier10.1016/j.vetpar.2014.04.025
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8880633
dc.descriptiontZoonotic visceral leishmaniasis (VL) is a widespread disease, and dogs are the main reser-voirs for human parasite transmission. Hence, development of an effective vaccine thatprevents disease and reduces the transmission of VL is required. As euthanasia of seropos-itive dogs is recommended in Brazil for VL epidemiological control, to include anti-VLcanine vaccines as a mass control measure it is necessary to characterize the humoralresponses induced by vaccination and if they interfere with the reactivity of vaccinateddogs in serological diagnostic tests. Leish-Tec®is an amastigote-specific A2 recombinantprotein vaccine against canine visceral leishmaniasis (CVL) that is commercially availablein Brazil. Here, we tested the immunogenicity of Leish-Tec®in a heterogeneous dog popula-tion by measuring A2-specific antibody responses. Healthy dogs (n = 140) of various breedswere allocated to two groups: one group received Leish-Tec®(n = 70), and the other groupreceived a placebo (n = 70). Anti-A2 or anti-Leishmania promastigote antigen (LPA) antibodylevels were measured by ELISA in serum samples collected before and after vaccination.An immunochromatographic test (DPP) based on the recombinant K28 antigen was alsoused for serodiagnosis of CVL. Vaccinated animals, except one, remained seronegative foranti-LPA total IgG and anti-K28 antibodies. Conversely, seropositivity for anti-A2 total IgGantibodies was found in 98% of animals after vaccination. This value decreased to 81.13% at6 months before rising again (98%), after the vaccination boost. Anti-A2 IgG2 and IgG1 titers∗
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectVisceral leishmaniasis
dc.subjectVaccine
dc.subjectLeish-Tec®
dc.subjectDogs
dc.subjectA2 antigen
dc.subjectAntibodies
dc.titleAntibody responses induced by Leish-Tec®, an A2-based vaccine for visceral leishmaniasis, in a heterogeneous canine population Miriam
dc.typeArticle


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