Cytosolic phospholipase A2-driven PGE2 synthesis within unsaturated fatty acids-induced lipid bodies of epithelial cells

dc.creatorMoreira, Luciana S.
dc.creatorPiva, Bruno
dc.creatorGentile, Luciana B.
dc.creatorSantos, Fabio P. Mesquita
dc.creatorD'Avila, Heloisa
dc.creatorMaya-Monteiro, Clarissa M.
dc.creatorBozza, Patrícia T.
dc.creatorMelo, Christianne Bandeira
dc.creatorDiaz, Bruno L.
dc.date2019-01-17T10:44:08Z
dc.date2019-01-17T10:44:08Z
dc.date2009
dc.date.accessioned2023-09-26T22:32:37Z
dc.date.available2023-09-26T22:32:37Z
dc.identifierMOREIRA, Luciana S. et al. Cytosolic phospholipase A2-driven PGE2 synthesis within unsaturated fatty acids-induced lipid bodies of epithelial cells. Biochimica et Biophysica Acta, v.1791, p.156–165, Jan. 2009.
dc.identifier1388-1981
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31088
dc.identifier10.1016/j.bbalip.2009.01.003
dc.identifier0006-3002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8880550
dc.descriptionCytoplasmic lipid bodies (also known as lipid droplets) are intracellular deposits of arachidonic acid (AA), which can be metabolized for eicosanoid generation. PGE2 is a major AA metabolite produced by epithelial cells and can modulate restoration of epithelium homeostasis after injury. We studied lipid body biogenesis and their role in AA metabolic pathway in an epithelial cell line derived from normal rat intestinal epithelium, IEC-6 cells. Lipid bodies were virtually absent in confluent IEC-6 cells. Stimulation of confluent IEC-6 cells with unsaturated fatty acids, including AA or oleic acid (OA), induced rapid lipid body assembly that was independent on its metabolism to PGE(2), but dependent on G-coupled receptor-driven signaling through p38, PKC, and PI3 K. Newly formed lipid bodies compartmentalized cytosolic phospholipase (cPL)A(2)-alpha, while facilitated AA mobilization and synthesis of PGE(2) within epithelial cells. Thus, both lipid body-related events, including highly regulated biogenesis and functional assembly of cPLA (2)-alpha-driven enhanced AA mobilization and PGE(2)production, may have key roles in epithelial cell-driven inflammatory functions, and may represent relevant therapeutic targets of epithelial pathologies.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectGotícula lipídica
dc.subjectCélula epitelial
dc.subjectÁcido araquidônico
dc.subjectÁcido oleico
dc.subjectLipid droplet
dc.subjectEpithelial cell
dc.subjectcPLA2-α
dc.subjectPGE2
dc.subjectArachidonic acid
dc.subjectOleic acid
dc.subjectp38 MAP kinase
dc.titleCytosolic phospholipase A2-driven PGE2 synthesis within unsaturated fatty acids-induced lipid bodies of epithelial cells
dc.typeArticle


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