Activation of Bothrops jararaca snake venom gland and venom production: a proteomic approach

dc.creatorLuna, Milene Schmidt
dc.creatorValente, Richard Hemmi
dc.creatorPerales, Jonas
dc.creatorVieira, Mônica Larucci
dc.creatorYamanouye, Norma
dc.date2016-07-21T11:42:05Z
dc.date2016-07-21T11:42:05Z
dc.date2013
dc.date.accessioned2023-09-26T22:32:25Z
dc.date.available2023-09-26T22:32:25Z
dc.identifierLUNA, Milene Schmidt; et al. Activation of Bothrops jararaca snake venom gland and venom production: A proteomic approach. Journal of Proteomics, v.94, p.460-472, Oct. 2013.
dc.identifier1874-3919
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15018
dc.identifier10.1016/j.jprot.2013.10.026
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8880511
dc.descriptionViperidae venom glands have a basal-central lumen where the venom produced by secretory cells is stored. We have shown that the protein composition of venom gland changes during the venom production cycle. Here, we analyzed the venom gland proteins during the venom production cycle by proteomic approach. We identified specific proteins in each stage of the cycle. Protein species from endoplasmic reticulum (PDI and GPR78) and cytoplasm (actin, vimentin, tropomyosin, proteasome subunit alpha type-1, thioredoxin, and 40S ribosomal protein) are more abundant in the activated stage, probably increasing the synthesis and secretion of toxins. We also showed for the first time that many toxins are present in the secretory cells during the quiescent stage. C-type lectin-like and serine proteinases were more abundant in the quiescent stage, and GPIb-BP and coagulation factor IX/X were present only in this stage. Metalloproteinases, L-amino acid oxidases, PLA2 and snake venom metalloproteinase and PLA2 inhibitors, and disintegrins were more abundant in the activated stage. Regarding metalloproteinases, the presence of peptides corresponding to the pro-domain was observed. These results allow us to better understand the mechanism of venom gland activation and venom production, contributing to studies about snake toxins and their diversity.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectSerpentes
dc.subjectGlândula de veneno
dc.subjectSíntese proteíca
dc.subjectCiclo de produção de veneno
dc.subjectAnálise proteômica
dc.subjectVenom gland
dc.subjectProtein synthesis
dc.subjectVenom production cycle
dc.subjectProteomic analysis
dc.subjectSnake
dc.titleActivation of Bothrops jararaca snake venom gland and venom production: a proteomic approach
dc.typeArticle


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