Stimulation of early eosinophil progenitors by a heat stable alveolar macrophage product from ovalbumin-sensitized and non-sensitized guinea pigs

dc.creatorElsas, Maria Ignez Capella Gaspar
dc.creatorXavier-Elsas, Pedro Paulo
dc.creatorJoseph, D.
dc.creatorHavet, N.
dc.creatorSilva, L. A. Adelino da
dc.creatorSalgado, D. R.
dc.creatorSaliou, B.
dc.creatorVargaftig, B. B.
dc.date2013-03-08T16:27:53Z
dc.date2013-03-08T16:27:53Z
dc.date1997
dc.date.accessioned2023-09-26T22:31:36Z
dc.date.available2023-09-26T22:31:36Z
dc.identifierELSAS, M. I, C, Stimulation of early eosinophil progenitors by a heat stable alveolar macrophage product from ovalbumin-sensitized and non-sensitized guinea pigs. Clin. exp. allergy., Oxford, v. 27, n. 2, p. 208-217, 1997.
dc.identifier1365-2222
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/6358
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8880354
dc.descriptionCNPq/RHAE, FINEP, PAPES/FIOCRUZ, CAPES-COFECUB e INSERM/Institut Pasteur
dc.descriptionBACKGROUND: Alveolar macrophages (AM) may participate in brochopulmonary hyper-reactivity by secreting cytokines that recruit mature eosinophils, or induce eosinophil production from recruited circulating progenitors. OBJECTIVE: To define whether AM products can contribute to lung eosinophil production in immunized guinea pigs (GP), by analysing the effect of AM culture supernatants (AM-SN) on in vitro eosinophilopoiesis. METHODS: Liquid and semi-solid bone marrow (BM) cultures were seeded with SN from 95% pure AM exposed to LPS. RESULTS: AM-SN increased very significantly the long-term viability, cell proliferation and eosinophil production in liquid culture and supported formation of eosinophil-bearing mixed colonies, by acting on progenitors depleted of mature eosinophils. The effect on eosinophil production was not duplicated by natural or recombinant sources of GM-CSF (which nevertheless supported GM colony formation by GP BM), not by rhIL-8 (which was active on GP cells) and was not due to residual LPS. FPLC separation of active AM SN yielded a peak of apparent m.w. 43 kDa, active on both liquid and semi-solid cultures. The active moiety was heat- and trypsin-resistant. Neutralizing monoclonal antibodies to hGM-CSF, mGM-CSF, hIL-3 and mIL-3 failed to deplete the activity in AM-SN. Ovalbumin immunization induced its production by AM even without LPS challenge. CONCLUSIONS: The lack of T lymphocytes among factor-producing AM, the properties of the active material, the inability of GM-CSF to reproduce these effects, and the failure of MoAbs to GM-CSF and to IL-3 to neutralize the activity indicate it is not due to the major eosinopoietic factors GM-CSF, IL-3 or IL-5.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBlackwell Scientific Publications
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dc.rightsrestricted access
dc.subjectEosinophil.
dc.subjectBone Marrow
dc.subjectAlveolar Macrophage
dc.subjectEosinófilos
dc.subjectCélulas-Tronco Hematopoéticas
dc.subjectMacrófagos Alveolares
dc.subjectOvalbumina
dc.titleStimulation of early eosinophil progenitors by a heat stable alveolar macrophage product from ovalbumin-sensitized and non-sensitized guinea pigs
dc.typeArticle


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