Trypanosoma evansi: molecular homogeneity as inferred by phenetical analysis of ribosomal internal transcribed spacers DNA of an eclectic parasite

dc.creatorLima, Aneska Norek de Oliveira
dc.creatorSantos, Simone da Silva
dc.creatorHerrera, Heitor Miraglia
dc.creatorGama, Carla
dc.creatorCupolillo, Elisa
dc.creatorJansen, Ana Maria
dc.creatorFernandes, Octávio
dc.date2018-08-23T16:21:55Z
dc.date2018-08-23T16:21:55Z
dc.date2008
dc.date.accessioned2023-09-26T22:31:13Z
dc.date.available2023-09-26T22:31:13Z
dc.identifierLIMA, Aneska Norek de Oliveira; et al. Trypanosoma evansi: Molecular homogeneity as inferred by phenetical analysis of ribosomal internal transcribed spacers DNA of an eclectic parasite. Experimental Parasitology, v.118, n.3, p.402-407, 2008.
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28334
dc.identifier10.1016/j.exppara.2007.10.003
dc.identifier1090-2449
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8880270
dc.descriptionThe protozoan Trypanosoma evansi is described as presenting high morphological and genetic similarities among the isolates despite its biological heterogeneity and wide geographical distribution. PCR amplification of the internal transcribed spacers of the ribosomal gene in combination with the coding region of the 5.8S ribosomal subunit further submitted to restriction enzymes digestion were carried out in DNAs extracted from 41 T. evansi strains isolated from horses, dogs, coatis and capybaras from two distinct regions of the Brazilian Pantanal. We also used one T. evansi isolate from Africa, one from Asia and one isolate of T. b. brucei from Africa. Analysis of the RFLP profiles yielded a unique "riboprinting" that does not vary intraspecifically. These results provide insights on the ribosomal gene organization of T. evansi and showed that ITS analysis by RFLP show high genetic similarity of this locus among isolates of this protozoan parasite.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectTrypanosoma evansi
dc.subjectReação em Cadeia da Polimerase
dc.subjectHomogeneidade molecular
dc.subjectanálise fenética de DNA
dc.subjectparasita eclético
dc.subjectespaçadores transcritos internos ribossômicos
dc.subjectTrypanosoma evansi
dc.subjectPCR
dc.subjectMolecular homogeneity
dc.subjectphenetical analysis
dc.subjecteclectic parasite
dc.subjectribosomal DNA
dc.subjectinternal transcribed spacer
dc.titleTrypanosoma evansi: molecular homogeneity as inferred by phenetical analysis of ribosomal internal transcribed spacers DNA of an eclectic parasite
dc.typeArticle


Este ítem pertenece a la siguiente institución