Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi

dc.creatorSantos, Isis Fernandes Magalhães
dc.creatorAndrade, Sonia Gumes
dc.date2011-05-11T20:47:14Z
dc.date2011-05-11T20:47:14Z
dc.date2005
dc.date.accessioned2023-09-26T22:26:46Z
dc.date.available2023-09-26T22:26:46Z
dc.identifierMAGALHÃES-SANTOS, I. F.; ANDRADE, S. G. Clinics in Sports Medicine. Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.100, n. 5, p. 555-561. aug., 2005
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/2653
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8879313
dc.descriptionCalomys callosus, a sylvatic reservoir of Trypanosoma cruzi, when infected with the Colombian strain (Biodeme Type III, T. cruzi I ) develops necrotic-inflammatory lesions and intense early fibrogenesis in the heart and skeletal muscles, that spontaneously regress. Participation of pro-inflammatory and pro-fibrogenic cytokines, such as tumor necrosis factor-alpha (TNF-α), gamma interferon (IFN-γ) , and tumor growth factor-beta (TGF-β), in the pathogenesis of the lesions is herein studied. Eighty C. callosus weighing 20 to 30 g were used. Seventy of them were inoculated with the Colombian strain (105 blood forms) and 10 were maintained as intact non-infected controls. After infection, C. callosus were sacrificed at different time-points from 15 to 70 days. The heart and skeletal muscle were processed for histopathology and cryopreserved for immunohistochemistry. Early necrotic lesions of parasitized skeletal muscle and myocardium with intense inflammatory lesions were present. Search for the in situ presence of TNF-α and IFN-γ, was performed using rat-IgG anti-mouse antibodies against these cytokines. For the in situ search of TGF-β, rabbit IgG anti-mouse antibodies were used. Immunolabeling of the cytokines in tissues of infected C. callosus was successful. The cytokines TNF-α, IFN-γ , and TGF-β were detected in the cytoplasm of macrophages and in the necrotic material from 15 to 45 days post-infection, decreasing their intensity until complete disappearance by the 65th day, which correlated with subsiding histopathological lesions. These findings suggest the participation of these cytokines in the control of parasite multiplication, in the development of an early fibrogenesis and in the regression of fibrotic-inflammatory lesions observed in C. callosus.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectCalomys callosus
dc.subjectCytokines
dc.subjectTumor necrosis factor-alpha
dc.subjectGamma interferon
dc.subjectTumor growth factor-beta
dc.subjectDoença de Chagas
dc.subjectCitocinas
dc.subjectMúsculo Esquelético
dc.subjectTrypanosoma cruzi
dc.subjectMiocárdio
dc.subjectFator Transformador de Crescimento beta
dc.subjectFator de Necrose Tumoral alfa
dc.subjectInterferon gama
dc.titleParticipation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi
dc.typeArticle


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