Clonal Dissemination of OXA-370-Producing Klebsiella pneumoniae in Rio de Janeiro, Brazil

dc.creatorPereira, Polyana Silva
dc.creatorBorghi, Miria
dc.creatorAraújo, Carlos Felipe Machado de
dc.creatorAires, Caio Augusto Martins
dc.creatorOliveira, Jane Cleide Ribeiro
dc.creatorAsensi, Marise Dutra
dc.creatorAssef, Ana Paula D’Alincourt Carvalho
dc.date2016-03-10T14:33:42Z
dc.date2016-03-10T14:33:42Z
dc.date2015
dc.date.accessioned2023-09-26T22:26:35Z
dc.date.available2023-09-26T22:26:35Z
dc.identifierPEREIRA, Polyana Silva; etal. Clonal Dissemination of OXA-370-Producing Klebsiella pneumoniae in Rio de Janeiro, Brazil. Antimicrobial Agents and Chemotherapy, v.59, n.8, p.4453-4456, Aug. 2015.
dc.identifier0066-4804
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/13097
dc.identifier10.1128/AAC.04243-14
dc.identifier1098-6596
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8879270
dc.descriptionEnzymes of the OXA-48 family have become some of the most important beta-lactamases in the world. A new OXA-48 variant (OXA-370) was first described for an Enterobacter hormaechei strain isolated in Rio Grande do Sul (southern region of Brazil) in 2013. Here we report detection of the blaOXA-370 gene in 24 isolates belonging to three Enterobacteriaceae species (22 Klebsiella pneumoniae isolates, 1 Enterobacter cloacae isolate, and 1 Enterobacter aerogenes isolate) collected from five hospitals in Rio de Janeiro, Brazil, in 2013 and 2014. The isolates showed a multidrug resistance profile, and 12.5% were resistant to polymyxin B. Besides blaOXA-370, no other carbapenemase genes were observed by PCR, whereas blaOXA-1 was found in all isolates and 22 isolates (91.6%) possessed blaCTX-M-15. Molecular typing of the K. pneumoniae isolates by pulsed-field gel electrophoresis (PFGE) showed the presence of two clonal groups, i.e., KpA (21 isolates) and KpB (1 isolate). KpA was characterized as sequence type 16 (ST16) and KpB as ST1041 by multilocus sequence typing (MLST). ST16 has been observed for KPC-producing K. pneumoniae in Rio de Janeiro. Plasmid analysis performed with six representative OXA-370-producing isolates showed plasmids harboring the blaOXA-370 gene in all strains, ranging from 25 kb to 150 kb. This study suggests that there is an urgent need to investigate the presence of OXA-370 and dissemination of the K. pneumoniae ST16 clone carrying this gene in Brazil.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectClonal Dissemination
dc.subjectOXA-370-Producing
dc.subjectKlebsiella pneumoniae
dc.subjectRio de Janeiro
dc.subjectEnterobacteriaceae
dc.subjectKlebsiella pneumoniae
dc.subjectbeta-Lactamases
dc.subjectInfecções por Enterobacteriaceae
dc.titleClonal Dissemination of OXA-370-Producing Klebsiella pneumoniae in Rio de Janeiro, Brazil
dc.typeArticle


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