Inhibition of HIV-1 infection by monoclonal antibodies to carbohydrates of Schistosoma mansoni.

dc.creatorMello, Marco Antônio Gomes
dc.creatorMascarenhas, Rita Elizabeth Moreira
dc.creatorFerraro, Geraldo Argolo
dc.creatorHarn Junior, Donald A
dc.creatorCastro Filho, Bernardo Galvão
dc.creatorBou-Habib, Dumith Chequer
dc.date2012-03-23T21:31:30Z
dc.date2012-03-23T21:31:30Z
dc.date2005
dc.date.accessioned2023-09-26T22:26:03Z
dc.date.available2023-09-26T22:26:03Z
dc.identifierMELLO, M.A. et al. Cinhibition of HIV-1 infection by monoclonal antibodies to carbohydrates of Schistosoma mansoni. Medical Microbiology Immunology, v. 194, n.1-2, p. 61-5, jan. 2005.
dc.identifier0300-8584
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/3813
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8879156
dc.descriptionPatients infected with HIV-1 develop a potent humoral immune response against the virus, but HIV-1 primary isolates are remarkably resistant to neutralizing antibodies. Considering that the envelope glycoprotein of HIV-1 (gp120/41) is heavily glycosylated, we investigated whether anti-carbohydrate antibodies could inhibit HIV-1 infection in vitro. We studied the neutralizing activity of three monoclonal antibodies (mAbs) raised to carbohydrates of Schistosoma mansoni, against seven primary isolates of HIV-1. Assays were performed infecting peripheral blood mononuclear cells from normal donors with viral isolates previously treated with mAbs. Viral strains used were tropic for the coreceptors CCR5, CXCR4, and dual-tropic ones. We found that the anti-glycan mAbs vigorously inhibited HIV-1 infection, regardless of the preferential coreceptor usage of the isolate, in a dose-response manner. Importantly, five isolates were resistant to neutralization by two HIV-1 antibody-positive human sera endowed with potent anti-HIV-1 inhibitory activity. Our findings suggest that carbohydrates of the HIV-1 viral envelope may be a target of an effective humoral immune response elicited by vaccination.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectHIV-1
dc.subjectAids
dc.subjectNeutralizing
dc.subjectAntibodies
dc.subjectAnticorpos Anti-Helmínticos
dc.subjectAnticorpos Monoclonais
dc.subjectCarboidratos
dc.subjectInfecções por HIV
dc.subjectSchistosoma mansoni
dc.subjectAntígenos de Helmintos
dc.subjectInfecções por HIV
dc.subjectHIV
dc.subjectTestes de Neutralização
dc.subjectAnimais
dc.titleInhibition of HIV-1 infection by monoclonal antibodies to carbohydrates of Schistosoma mansoni.
dc.typeArticle


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