Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells

dc.creatorStumbo, Ana Carolina
dc.creatorBarbosa, Helene Santos
dc.creatorCarvalho, Técia Maria Ulisses de
dc.creatorPorto, Luís Cristóvão
dc.creatorCarvalho, Laís de
dc.date2019-07-23T13:25:55Z
dc.date2019-07-23T13:25:55Z
dc.date2002
dc.date.accessioned2023-09-26T22:25:55Z
dc.date.available2023-09-26T22:25:55Z
dc.identifierSTUMBO, Ana Carolina et al. Anionic Sites, Fucose Residues and Class I Human Leukocyte Antigen Fate During Interaction of Toxoplasma gondii with Endothelial Cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 4, p. 517-522, June 2002.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/34343
dc.identifier10.1590/s0074-02762002000400012
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8879126
dc.descriptionToxoplasma gondii invades and proliferates in human umbilical vein endothelial cells where it resides in a parasitophorous vacuole. In order to analyze which components of the endothelial cell plasma membrane are internalized and become part of the parasitophorous vacuole membrane, the culture of endothelial cells was labeled with cationized ferritin or UEA I lectin or anti Class I human leukocyte antigen (HLA) before or after infection with T. gondii. The results showed no cationized ferritin and UEA I lectin in any parasitophorous vacuole membrane, however, the Class I HLA molecule labeling was observed in some endocytic vacuoles containing parasite until 1 h of interaction with T. gondii. After 24 h parasite-host cell interaction, the labeling was absent on the vacuolar membrane, but presents only in small vesicles near parasitophorous vacuole. These results suggest the anionic site and fucose residues are excluded at the time of parasitophorous vacuole formation while Class I HLA molecules are present only on a minority of Toxoplasma-containing vacuoles.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectToxoplasma gondii
dc.subjectCélulas Endoteliais
dc.subjectFerritinas
dc.subjectAntígenos de Histocompatibilidade Classe I
dc.subjectAntígenos de Histocompatibilidade Classe 1
dc.subjectToxoplasma gondii
dc.subjectEndothelial cells
dc.subjectFerritin
dc.subjectUlex europaeus agglutinin-1
dc.subjectHistocompatibility antigens Class I
dc.subjectCélulas Endoteliais
dc.subjectAntígenos de Histocompatibilidade Classe I
dc.subjectAntígenos de Histocompatibilidade
dc.titleAnionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells
dc.typeArticle


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