Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil

dc.creatorCalegar, Deiviane Aparecida
dc.creatorNunes, Beatriz Coronato
dc.creatorMonteiro, Kerla Joeline Lima
dc.creatorSantos, Jéssica Pereira dos
dc.creatorToma, Helena Keiko
dc.creatorGomes, Tais Ferreira
dc.creatorLima, Marli Maria
dc.creatorBóia, Márcio Neves
dc.creatorCarvalho-Costa, Filipe Anibal
dc.date2016-06-23T18:50:16Z
dc.date2016-07-07T11:59:19Z
dc.date2016-06-23T18:50:16Z
dc.date2016-07-07T11:59:19Z
dc.date2016
dc.date.accessioned2023-09-26T22:25:43Z
dc.date.available2023-09-26T22:25:43Z
dc.identifierCALEGAR, Deiviane Aparecida; et al. Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil. Mem Inst Oswaldo Cruz, v.111, n.2, p.114-119, Feb. 2016.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14642
dc.identifier10.1590/0074-02760150383
dc.identifier1678-8080
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8879080
dc.descriptionThis study aimed to estimate the frequency, associated factors, and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni infections. We performed a survey (n = 213 subjects) to obtain parasitological, sanitation, and sociodemographic data. Faecal samples were processed through flotation and centrifugation methods. E. histolytica, E. dispar, E. moshkovskii, and E. hartmanni were identified by nested-polymerase chain reaction (PCR). The overall prevalence of infection was 22/213 (10.3%). The infection rate among subjects who drink rainwater collected from roofs in tanks was higher than the rate in subjects who drink desalinated water pumped from wells; similarly, the infection rate among subjects who practice open defecation was significantly higher than that of subjects with latrines. Out of the 22 samples positive for morphologically indistinguishable Entamoeba species, the differentiation by PCR was successful for 21. The species distribution was as follows: 57.1% to E. dispar, 23.8% to E. histolytica, 14.3% to E. histolytica and E. dispar, and 4.8% E. dispar and E. hartmanni. These data suggest a high prevalence of asymptomatic infection by the group of morphologically indistinguishable Entamoeba histolytica/dispar/moshkovskii complex and E. hartmanni species. In this context of water scarcity, the sanitary and socioenvironmental characteristics of the region appear to favour transmission.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz
dc.rightsopen access
dc.subjectParasitos
dc.subjectParasitologia
dc.subjectEntamebíase
dc.subjectEpidemiologia
dc.subjectBrasil
dc.subjectParasites
dc.subjectProtozoa
dc.subjectentamoebiasis
dc.subjectEpidemiology
dc.subjectBrazil
dc.titleFrequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
dc.typeArticle


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