| dc.description | Fibroblast growth factor 23 (FGF23) is an endocrine growth factor and
known to play a pivotal role in phosphate homeostasis. Interestingly, several
studies point towards a function of FGF23 in the hypothalamus. FGF23 clas sically activates the FGF receptor 1 in the presence of the co-receptor
αKlotho, of both gene expression in the brain was previously established.
However, studies on gene and protein expression of FGF23 in the brain are
scarce and have been inconsistent. Therefore, our aim was to localise FGF23
gene and protein expression in the rat brain with focus on the hypothalamus.
Also, we investigated the protein expression of αKlotho. Adult rat brains
were used to localise and visualise FGF23 and αKlotho protein in the hypo thalamus by immunofluorescence labelling. Furthermore, western blots were
used for assessing hypothalamic FGF23 protein expression. FGF23 gene
expression was investigated by qPCR in punches of the arcuate nucleus, lat eral hypothalamus, paraventricular nucleus, choroid plexus, ventrolateral
thalamic nucleus and the ventromedial hypothalamus. Immunoreactivity for
FGF23 and αKlotho protein was found in the hypothalamus, third ventricle
lining and the choroid plexus. Western blot analysis of the hypothalamus Fibroblast growth factor 23 (FGF23) is an endocrine growth factor and
known to play a pivotal role in phosphate homeostasis. Interestingly, several
studies point towards a function of FGF23 in the hypothalamus. FGF23 clas sically activates the FGF receptor 1 in the presence of the co-receptor
αKlotho, of both gene expression in the brain was previously established.
However, studies on gene and protein expression of FGF23 in the brain are
scarce and have been inconsistent. Therefore, our aim was to localise FGF23
gene and protein expression in the rat brain with focus on the hypothalamus.
Also, we investigated the protein expression of αKlotho. Adult rat brains
were used to localise and visualise FGF23 and αKlotho protein in the hypo thalamus by immunofluorescence labelling. Furthermore, western blots were
used for assessing hypothalamic FGF23 protein expression. FGF23 gene
expression was investigated by qPCR in punches of the arcuate nucleus, lat eral hypothalamus, paraventricular nucleus, choroid plexus, ventrolateral
thalamic nucleus and the ventromedial hypothalamus. Immunoreactivity for
FGF23 and αKlotho protein was found in the hypothalamus, third ventricle
lining and the choroid plexus. Western blot analysis of the hypothalamusFibroblast growth factor 23 (FGF23) is an endocrine growth factor and
known to play a pivotal role in phosphate homeostasis. Interestingly, several
studies point towards a function of FGF23 in the hypothalamus. FGF23 clas sically activates the FGF receptor 1 in the presence of the co-receptor
αKlotho, of both gene expression in the brain was previously established.
However, studies on gene and protein expression of FGF23 in the brain are
scarce and have been inconsistent. Therefore, our aim was to localise FGF23
gene and protein expression in the rat brain with focus on the hypothalamus.
Also, we investigated the protein expression of αKlotho. Adult rat brains
were used to localise and visualise FGF23 and αKlotho protein in the hypo thalamus by immunofluorescence labelling. Furthermore, western blots were
used for assessing hypothalamic FGF23 protein expression. FGF23 gene
expression was investigated by qPCR in punches of the arcuate nucleus, lat eral hypothalamus, paraventricular nucleus, choroid plexus, ventrolateral
thalamic nucleus and the ventromedial hypothalamus. Immunoreactivity for
FGF23 and αKlotho protein was found in the hypothalamus, third ventricle
lining and the choroid plexus. Western blot analysis of the hypothalamusconfirmed the presence of FGF23. Gene expression of FGF23 was not
detected, suggesting that the observed FGF23 protein is not brain-derived.
Several FGF receptors are known to be present in the brain. Therefore, we
conclude that the machinery for FGF23 signal transduction is present in sev eral brain areas, indeed suggesting a role for FGF23 in the brain. | |
