Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds

dc.creatorMendes, Graziele
dc.creatorGonçalves, Vivian Nicolau
dc.creatorFagundes, Elaine Maria de Souza
dc.creatorKohlhoff, Markus
dc.creatorRosa, Carlos Augusto
dc.creatorZani, Carlos Leomar
dc.creatorCota, Betania Barros
dc.creatorRosa, Luiz Henrique
dc.creatorJohann, Susana
dc.date2016-08-22T14:05:49Z
dc.date2016-08-22T14:05:49Z
dc.date2016
dc.date.accessioned2023-09-26T22:25:16Z
dc.date.available2023-09-26T22:25:16Z
dc.identifierMENDES, Graziele et al. Antifungal activity of extracts from Atacama Desert fungi againstParacoccidioides brasiliensis and identification ofAspergillus felis as a promising source of natural bioactive compounds. Mem. Inst. Oswaldo Cruz, v. 111, n. 3, p. 209-217, 2016.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15316
dc.identifier10.1590/0074-02760150451
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878982
dc.descriptionFungi of the genus Paracoccidioides are responsible for paracoccidioidomycosis. The occurrence of drug toxicity and relapse in this disease justify the development of new antifungal agents. Compounds extracted from fungal extract have showing antifungal activity. Extracts of 78 fungi isolated from rocks of the Atacama Desert were tested in a microdilution assay against Paracoccidioides brasiliensis Pb18. Approximately 18% (5) of the extracts showed minimum inhibitory concentration (MIC) values≤ 125.0 µg/mL. Among these, extract from the fungus UFMGCB 8030 demonstrated the best results, with an MIC of 15.6 µg/mL. This isolate was identified as Aspergillus felis (by macro and micromorphologies, and internal transcribed spacer, β-tubulin, and ribosomal polymerase II gene analyses) and was grown in five different culture media and extracted with various solvents to optimise its antifungal activity. Potato dextrose agar culture and dichloromethane extraction resulted in an MIC of 1.9 µg/mL against P. brasiliensis and did not show cytotoxicity at the concentrations tested in normal mammalian cell (Vero). This extract was subjected to bioassay-guided fractionation using analytical C18RP-high-performance liquid chromatography (HPLC) and an antifungal assay using P. brasiliensis. Analysis of the active fractions by HPLC-high resolution mass spectrometry allowed us to identify the antifungal agents present in the A. felis extracts cytochalasins. These results reveal the potential of A. felis as a producer of bioactive compounds with antifungal activity.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz
dc.rightsopen access
dc.subjectRock-inhabiting Fungi
dc.subjectAtacama Desert
dc.subjectParacoccidioides brasiliensis
dc.subjectAntifungal
dc.subjectAspergillus Felis
dc.titleAntifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds
dc.typeArticle


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