Reciprocal immune enhancement of dengue and Zika virus infection in human skin

dc.creatorCastanha, Priscila M. S.
dc.creatorErdos, Geza
dc.creatorWatkins, Simon C.
dc.creatorFalo Jr., Louis D.
dc.creatorMarques, Ernesto T. A.
dc.creatorBarratt-Boyes, Simon M.
dc.date2021-05-20T16:29:38Z
dc.date2021-05-20T16:29:38Z
dc.date2020
dc.date.accessioned2023-09-26T22:24:24Z
dc.date.available2023-09-26T22:24:24Z
dc.identifierCASTANHA, P. M. S. et al. Reciprocal immune enhancement of dengue and Zika virus infection in human skin. JCI insight, v. 5, n. 3, 13 fev. 2020.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/47276
dc.identifier10.1172/jci.insight.133653
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878852
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior Pessoal / Ministério da Educação do Brasil (Programa Nacional de Pós-doutorado), National Institutes of Health.
dc.descriptionDengue virus (DENV) and Zika virus (ZIKV) are closely related mosquito-borne flaviviruses that co-circulate in tropical regions and constitute major threats to global human health. Whether preexisting immunity to one virus affects disease caused by the other during primary or secondary infections is unknown but is critical in preparing for future outbreaks and predicting vaccine safety. Using a human skin explant model, we show that DENV-3 immune sera increased recruitment and infection of Langerhans cells, macrophages, and dermal dendritic cells following inoculation with DENV-2 or ZIKV. Similarly, ZIKV immune sera enhanced infection with DENV-2. Immune sera increased migration of infected Langerhans cells to the dermis and emigration of infected cells out of skin. Heterotypic immune sera increased viral RNA in the dermis almost 10-fold and reduced the amount of virus required to infect a majority of myeloid cells by 100- to 1000-fold. Enhancement was associated with cross-reactive IgG and induction of IL-10 expression and was mediated by both CD32 and CD64 Fcγ receptors. These findings reveal that preexisting heterotypic immunity greatly enhances DENV and ZIKV infection, replication, and spread in human skin. This relevant tissue model will be valuable in assessing the efficacy and risk of dengue and Zika vaccines in humans.
dc.description2026-12-30
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectCélulas Dendríticas
dc.subjectImunoglobulinas
dc.subjectImunologia
dc.subjectDoenças Transmissíveis
dc.subjectMacrófagos
dc.titleReciprocal immune enhancement of dengue and Zika virus infection in human skin
dc.typeArticle


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