Interleukin 10-Dominant Immune Response and Increased Risk of Cutaneous Leishmaniasis After Natural Exposure to Lutzomyia intermedia Sand Flies

dc.creatorCarvalho, Augusto M
dc.creatorCristal, Juqueline Rocha
dc.creatorMuniz, Aline de Couto
dc.creatorCarvalho, Lucas Pedreira de
dc.creatorGomes, Regis
dc.creatorMiranda, José Carlos
dc.creatorBarral, Aldina Maria Prado
dc.creatorCarvalho Filho, Edgar Marcelino
dc.creatorOliveira, Camila Indiani de
dc.date2015-03-09T17:51:38Z
dc.date2015-03-09T17:51:38Z
dc.date2015
dc.date.accessioned2023-09-26T22:24:23Z
dc.date.available2023-09-26T22:24:23Z
dc.identifierCARVALHO, A. M. et al. Interleukin 10-dominant immune response and increased risk of Cutaneous Leishmaniasis after natural exposure to Lutzomyia intermedia Sand Flies. Journal of Infectious Diseases, fevereiro 2015.
dc.identifier0022-1899
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/9665
dc.identifier10.1093/infdis/jiv020
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878850
dc.descriptionLeishmaniasis is caused by parasites transmitted to the vertebrate host by infected sand flies. During transmission, the vertebrate host is also inoculated with sand fly saliva, which exerts powerful immunomodulatory effects on the host’s immune response. Methods. We conducted a prospective cohort analysis to characterize the human immune response to Lutzomyia intermedia saliva in 264 individuals, from an area for cutaneous leishmaniasis (CL) caused by Leishmania braziliensis. Results. Antibodies were found in 150 individuals (56.8%); immunoglobulin G1 and G4 were the predominant subclasses. Recall responses to salivary gland sonicate showed elevated production of interleukin 10 (IL-10), interleukin 13, interferon γ, CXCL9, and CCL2 compared with controls. CD4+CD25+ T cells, including Foxp3+ cells, were the main source of IL-10. L. braziliensis replication was increased (P < .05) in macrophages cocultured with saliva-stimulated lymphocytes from exposed individuals and addition of anti–IL-10 reverted this effect. Positive correlation between antibody response to saliva and cellular response to Leishmania was not found. Importantly, individuals seropositive to saliva are 2.1 times more likely to develop CL (relative risk, 2.1; 95% confidence interval, 1.07–4.2; P < .05). Conclusions. Exposure to L. intermedia sand flies skews the human immune response, facilitating L. braziliensis survival in vitro, and increases the risk of developing CL.
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversity of Chicago Press
dc.rightsopen access
dc.subjectSand fly saliva
dc.subjectL. braziliensis
dc.subjectCutaneous leishmaniasis
dc.subjectLutzomyia intermedia
dc.subjectELISA
dc.subjectCytokines
dc.subjectChemokines
dc.titleInterleukin 10-Dominant Immune Response and Increased Risk of Cutaneous Leishmaniasis After Natural Exposure to Lutzomyia intermedia Sand Flies
dc.typeArticle


Este ítem pertenece a la siguiente institución