Distribution of Merlin in eukaryotes and first report of DNA transposons in kinetoplastid protists

dc.creatorLopes, Ana Luisa Kalb
dc.creatorKriegova, Eva
dc.creatorLukes, Julius
dc.creatorKrieger, Marco Aurélio
dc.creatorLudwig, Adriana
dc.date2021-06-11T15:21:06Z
dc.date2021-06-11T15:21:06Z
dc.date2021
dc.date.accessioned2023-09-26T22:24:22Z
dc.date.available2023-09-26T22:24:22Z
dc.identifierLOPES, Ana Luisa Kalb et al. Distribution of Merlin in eukaryotes and first report of DNA transposons in kinetoplastid protists. PLoS ONE, v. 16, n. 5, p. 1-24, 2021.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/47649
dc.identifier10.1371/journal.pone.0251133
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878843
dc.descriptionDNA transposons are defined as repeated DNA sequences that can move within the host genome through the action of transposases. The transposon superfamily Merlin was originally found mainly in animal genomes. Here, we describe a global distribution of the Merlin in animals, fungi, plants and protists, reporting for the first time their presence in Rhodophyceae, Metamonada, Discoba and Alveolata. We identified a great variety of potentially active Merlin families, some containing highly imperfect terminal inverted repeats and internal tandem repeats. Merlin-related sequences with no evidence of mobilization capacity were also observed and may be products of domestication. The evolutionary trees support that Merlin is likely an ancient superfamily, with early events of diversification and secondary losses, although repeated re-invasions probably occurred in some groups, which would explain its diversity and discontinuous distribution. We cannot rule out the possibility that the Merlin superfamily is the product of multiple horizontal transfers of related prokaryotic insertion sequences. Moreover, this is the first account of a DNA transposon in kinetoplastid flagellates, with conserved Merlin transposase identified in Bodo saltans and Perkinsela sp., whereas it is absent in trypanosomatids. Based on the level of conservation of the transposase and overlaps of putative open reading frames with Merlin, we propose that in protists it may serve as a raw material for gene emergence.
dc.formatapplication/pdf
dc.languagepor
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectRhodophyceae
dc.subjectMetamonada
dc.subjectDiscoba
dc.subjectDNA Transposable Elements
dc.subjectSuperfamily Merlin
dc.subjectAlveolata
dc.subjectGenome
dc.subjectElementos Transponibles de ADN
dc.subjectÉléments transposables d'ADN
dc.subjectGénome
dc.subjectElementos de DNA Transponíveis
dc.subjectAlveolados
dc.subjectGenoma
dc.titleDistribution of Merlin in eukaryotes and first report of DNA transposons in kinetoplastid protists
dc.typeArticle


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