Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies

dc.creatorFranco, Antonia Maria Ramos
dc.creatorMachado, Gérzia M. C.
dc.creatorNaiff, Roberto D.
dc.creatorMoreira, Celia F. S.
dc.creatorMcMahon-Pratt, Diane
dc.creatorGrimaldi Jr., Gabriel
dc.date2020-10-07T12:57:12Z
dc.date2020-10-07T12:57:12Z
dc.date1997
dc.date.accessioned2023-09-26T22:23:22Z
dc.date.available2023-09-26T22:23:22Z
dc.identifierFRANCO, Antonia Maria Ramos et al. Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 1, p. 63-69, Jan./Feb. 1997.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43843
dc.identifier10.1590/S0074-02761997000100013
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878617
dc.descriptionA large number of Endotrypanum stocks (representing an heterogeneous population of strains) have been screened against a panel of monoclonal antibodies (MAbs) derived for selected species of Endotrypanum or Leishmania, to see whether this approach could be used to group/differentiate further among these parasites. Using different immunological assay systems, MAbs considered specific for the genus Endotrypanum (E-24, CXXX-3G5-F12) or strain M6159 of E. schaudinni (E-2, CXIV-3C7-F5) reacted variably according to the test used but in the ELISA or immunofluorescence assay both reacted with all the strains tested. Analyses using these MAbs showed antigenic diversity occurring among the Endotrypanum strains, but no qualitative or quantitative reactivity pattern could be consistently related to parasite origin (i.e., host species involved) or geographic area of isolation. Western blot analyses of the parasites showed that these MAbs recognized multiple components. Differences existed either in the epitope density or molecular forms associated with the antigenic determinants and therefore allowed the assignment of the strains to specific antigenic groups. Using immunofluorescence or ELISA assay, clone E-24 produced reaction with L. equatorensis (which is a parasite of sloth and rodent), but not with other trypanosomatids examined. Interestingly, the latter parasite and the Endotrypanum strains crossreacted with a number of MAbs that were produced against members of the L. major-L. tropica complex.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectEndotrypanum
dc.subjectKinetoplastid
dc.subjectTrypanosomatidae
dc.subjectReservatórios de mamíferos
dc.subjectCaracterização molecular
dc.subjectAnticorpos monoclonais
dc.subjectEndotrypanum
dc.subjectKinetoplastid
dc.subjectTrypanosomatidae
dc.subjectMammalian reservoirs
dc.subjectMolecular characterization
dc.subjectMonoclonal antibodies
dc.titleCharacterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
dc.typeArticle


Este ítem pertenece a la siguiente institución