Monitoring of total antimony and its species by ICP-MS and on-line ion chromatography in biological samples from patients treated for leishmaniasis.

dc.creatorMiekeley, N.
dc.creatorMortari, S. R.
dc.creatorSchubach, A.
dc.date2019-05-16T12:59:57Z
dc.date2019-05-16T12:59:57Z
dc.date2002
dc.date.accessioned2023-09-26T22:22:25Z
dc.date.available2023-09-26T22:22:25Z
dc.identifierMIEKELEY, N.; MORTARI, S. R.; SCHUBACH, A. Monitoring of total antimony and its species by ICP-MS and on-line ion chromatography in biological samples from patients treated for leishmaniasis. Analytical and Bioanalytical Chemistry, v. 372, p. 495–502, 2002.
dc.identifier0011-9059
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33106
dc.identifier10.1007/s00216-001-1213-7
dc.identifier1365-4632
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878433
dc.descriptionResults from a study are reported in which patients with leishmaniasis were monitored by whole blood, blood plasma, urine, and hair analysis, before, during, and after intramuscular administration of N-methyl meglumine antimoniate. Quadrupole ICP-MS was used for the detection of antimony and on-line ion chromatography for the separation of its species. After typically 30 consecutive daily injections of 5 mg antimony per kg of body weight, Sb concentrations of up to 250 µg L–1 in whole blood and plasma, and 60 mg of Sb per gram of creatinine in urine, were measured 24 h after drug administration. Antimony in hair samples of these patients showed concentrations of up to 24 µg g–1. Speciation studies of Sb5+ and Sb3+ in drug, urine, and plasma samples were performed by ion chromatography using a Hamilton PRP-100X anion exchange column and EDTA (2 or 20 mM, pH 4.7) as the mobile phases. Repeatability of elution time and peak area measurements for a 0.125 ng spike were <1.2% and <3.5%, respectively. Method detection limits for both species, using a 1:10 diluted urine or plasma sample, were typically 1.6 µg L–1. The procedure was capable of separating the very intense drug peak from its inorganic species, thus permitting the first studies on the bio-transformation of N-methyl meglumine antimoniate to Sb5+ and Sb3+ in the human body.
dc.description2020-05-16
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsopen access
dc.subjectAntimony speciation
dc.subjectIon chromatography
dc.subjectICP-MS
dc.subjectLeishmaniasis
dc.titleMonitoring of total antimony and its species by ICP-MS and on-line ion chromatography in biological samples from patients treated for leishmaniasis.
dc.typeArticle


Este ítem pertenece a la siguiente institución