Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen

dc.creatorvon Sydow, Farid F. O .
dc.creatorSantiago, Marta A.
dc.creatorSouza, Patricia C. Neves
dc.creatorCerqueira, Denise I. S .
dc.creatorGouvea, Adriana S.
dc.creatorLavatori, Maryrose F. H.
dc.creatorBertho, Álvaro L.
dc.creatorKubelka, Claire F.
dc.date2020-10-20T17:39:51Z
dc.date2020-10-20T17:39:51Z
dc.date2000
dc.date.accessioned2023-09-26T22:21:34Z
dc.date.available2023-09-26T22:21:34Z
dc.identifiervon Sydow, Farid F. O. et al. Comparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 4, p. 483-489, 2000.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44084
dc.identifier10.1590/S0074-02762000000400007
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878300
dc.descriptionFluorescent activated cell sorter (FACS) analysis is useful for the detection of cellular surface antigens and intracellular proteins. We used this methodology in order to detect and quantify dengue antigens in highly susceptible cells such as clone C6/36 (Aedes albopictus) and Vero cells (green monkey kidney). Additionally, we analyzed the infection in vitro of human peripheral blood mononuclear leukocytes (PBML). FACS analysis turned out to be a reliable technique to quantify virus growth in traditional cell cultures of C6/36 as well as Vero cells. High rates of infection were achieved with a good statistical correlation between the virus amount used in infection and the percentage of dengue antigen containing cells detected in infected cultures. We also showed that human monocytes (CD14+) are preferred target cells for in vitro dengue infection among PBML. Monocytes were much less susceptible to virus infection than cell lines but they displayed dengue antigens detected by FACS five days after infection. In contrast, lymphocytes showed no differences in their profile for dengue specific immunofluorescence. Without an animal model to reproduce dengue disease, alternative assays have been sought to correlate viral virulence with clinical manifestations and disease severity. Study of in vitro interaction of virus and host cells may highlight this relationship.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectVírus da Dengue
dc.subjectCitometria de fluxo
dc.subjectCélulas vero
dc.subjectLeucócitos mononucleares
dc.subjectC6/36
dc.subjectDengue virus
dc.subjectC6/36
dc.subjectVero cells
dc.subjectMononuclear leukocytes
dc.subjectFlow cytometry
dc.titleComparison of Dengue Infection in Human Mononuclear Leukocytes with Mosquito C6/36 and Mammalian Vero Cells Using Flow Cytometry to Detect Virus Antigen
dc.typeArticle


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